Sartobind® Q Cassette 0.8 L with 4 mm bed height

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.

Item No.: 
98IEXQ42D-L

*Custom/bulk order quotes are provided within 72 hours of request.

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Applications

  • Application
    Polishing  

Compliance Information

  • Biological Reactivity
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).  

Connections (Physical)

  • Connectors
    Sanitary 1" – 1.5" via manifold  

Dimensions

  • Bed Height
    4 mm  
  • Dimensions WxDxH
    634 × 49 × 387 mm  
  • Membrane Area
    2.9 m²  

Features

  • Reusable
    Yes  

Materials of Construction

  • Housing Material
    Acrylonitrile butadiene styrene (ABS)  
  • Materials of Construction
    Polyester  
  • Membrane Material
    Stabilized reinforced cellulose  

Operating Conditions

  • Maximum Operating Pressure
    0.2 MPa  
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi  
  • Recommended Flow Rate
    16 L/min  
  • pH Stability
    pH 2 – 14 (short term), pH 2 – 12 (long term)  

Physicochemical Information

  • Bed Volume
    0.8 L  
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents  
  • Ligand
    Quaternary ammonium  
  • Ligand Density
    2 – 5 µeq/cm²  
  • Pore Size
    3 – 5 µm  
  • Reference Protein
    Bovine serum albumin (BSA)  
  • Thermal Stability
    Sartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.  
  • Typical Dynamic Binding Capacity 10% per unit
    23.2 g  
  • Void Volume
    2.5 L  

Product Information

  • Brand
    Sartobind® Q  
  • Membrane Adsorber Format
    Cassettes  
  • Membrane Type
    Strong anion exchange membrane  
  • Pack Size
    1  
  • Type
    Membrane adsorber  
  • What you receive
    • 1 cassette  
    • 1 operating instruction  
    • 1 certificate  

Sterility/Purity

  • Sterilization Method
    Autoclavable  
  • Manifold Set for Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

    The manifold set contains the inlet and outlet adapter plates for Sartoclear® Depth Filters as well as Sartobind® membrane adsorbers cassettes.

  • Pilot Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

    Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.

  • Double Process Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

    Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.

  • Process Filter Holder — For Sartoclear® Depth Filters and Sartobind® Membrane Adsorbers

    Filter Holders are hydraulic clamping systems especially designed for the use of modular Sartoclear® Depth Filter cassettes and Sartobind® membrane chromatography cassettes. The holders are available in three standard formats for pilot scale and commercial manufacturing.