Vivaflow 200, 30,000 MWCO Hydrosart
0.5 - 5 l
- Plug and play laboratory cross flow cassette
- Re-usable
- Optimal for cell culture supernatant and virus concentration
- High flow rate and recovery
Item no.: VF20H2
Price: $460.00
Availability (ex works): For more information please log in
Choose your Vivaflow 200 Laboratory Cross Flow Cassette configuration
Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.
Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.
Equipment required
The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
Equipment required
The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
Running 2 Vivaflow® 200 cassettes in parallel
For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel.
- High flow rate and recovery
- Clear housing for process monitoring
- Ready to use single cassettes
- Easy set up of 2 cassettes for use in parallel
- Re-usable product
Performance Characteristics
| Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C | ||||
|---|---|---|---|---|
| Start volume | Average flux | Recovery % | ||
| 1 litre | ml/min | Direct | 25 ml rinse | |
| Lysozyme 0.25 mg/ml (14,000 MW) | ||||
| 2,000 MWCO Hydrosart | 160 | 6 | 97 % | >99 % |
| 3,000 MWCO PES | 180 | 5 | 97 % | >99 % |
| BSA 1.0 mg/ml (66,000 MW) | ||||
| 5,000 MWCO PES | 29 | 33 | 98 % | >99 % |
| 5,000 MWCO Hydrosart | 70 | 14 | 98 % | >99 % |
| 10,000 MWCO PES | 23 | 41 | 96 % | >99 % |
| 10,000 MWCO Hydrosart | 35 | 27 | 98 % | >99 % |
| 30,000 MWCO PES | 25 | 38 | 96 % | 99 % |
| 30,000 MWCO Hydrosart | 20 | 48 | 96 % | >99 % |
| 50,000 MWCO PES | 22 | 43 | 96 % | 98 % |
| γ Globulins 1.0 mg/ml (average 160,000 MW) | ||||
| 100,000 MWCO PES | 54 | 18 | 96 % | 99 % |
| 100,000 MWCO RC | 45 | 21 | 96 % | 99 % |
| Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
| 0.2 μm PES | 11 | 86 | 92 % | 98 % |
| Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES | ||||
| BSA 0.001 mg/ml | 18 | 52 | 90 % | 98 % |
| BSA 0.01 mg/ml | 20 | 47 | 92 % | 98 % |
| BSA 0.1 mg/ml | 21 | 45 | 94 % | 99 % |
| Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES | ||||
| BSA 1.0 mg/ml (66,000 MW) | 67 | 70 | 97 % | >99 % |
Application Note
Brochure
Catalog
Datasheet
User Manual
Applications
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ApplicationProtein Concentration
-
Area of Application
-
Buffer Exchange
- Protein/DNA Concentration
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Buffer Exchange
-
Market
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BioPharma
- Research
-
BioPharma
Certificates
-
CertificationNone
Compliance Information
-
Animal ComponentsNo
-
Electrical / Battery itemNo
Connections (Physical)
-
FittingsNylon (NY)
Dimensions
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Channel Height0.4 mm
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Channel Width10 mm
-
Membrane Area200 cm²
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Overall Height138 mm
-
Overall Length127 mm
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Width38 mm
Materials of Construction
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Flow ChannelAcrylic
-
Flow RestrictorPolypropylene (PP)
-
Housing MaterialAcrylic
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Material o-ring | sealSilicone (SI)
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Membrane MaterialHydrosart®
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Membrane SupportPolypropylene (PP)
-
Pressure IndicatorPolypropylene, SS spring
Operating Conditions
-
Maximum Allowable Working Temperature60 °C (140 °F)
-
Maximum Operating Pressure4 bar (58 psi)
Physicochemical Information
-
MWCO30 kD
-
Non-recoverable Hold-up< 2 mL
Product Information
-
Device TypeTangential / Crossflow Concentrator
-
Pack Size1
-
Primary Product TypeUltrafiltration Devices
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Sample Type
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Protein
-
Nanoparticles
- Virus
-
Protein
Specifications
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Pump Flow200 – 400 mL/min
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TubingPVC (medical grade)
Volumes
-
Hold-up Volume5.3 mL
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Minimum Recirculation Volume< 20 mL
-
Volume Range500 – 5,000 mL
Tubing Set for Vivaflow® 50
Comprises: 2 x 1 m size 16 PVC tubing with inlet fittings, 2 x 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 x series interconnector
Complete set of accessories for the Vivaflow® 50 with a 230 V pump
Comprises: Pump (230 V), Easy Load pump head (size 16), tubing, 500 ml sample| diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors
Complete set of accessories for the Vivaflow® 50 with a 115 V pump
Comprises: Pump (115 V), Easy Load pump head (size 16), tubing, 500 ml sample| diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors
Y connector (size 15 to 2 x size 16) for operating two Vivaflow® 200 units
Y Connector (size 15 to 2x size 16)
Female Luer fittings Size 16 (10 pieces) to be used with the Vivaflow® systems
Female luer fittings size 15 (10 pieces)
Female Luer fittings Size 15 to be used with the Vivaflow® systems
Masterflex Easy Load Pump Head - Size 16
Masterflex Easy Load Pump Head - Size 15
Pump for running the Vivaflow-unit
Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.
Complete set of accessories for the Vivaflow® 50R|200 with a 230 V pump
Comprises: Pump (230 V), Easy Load pump head (size 16), tubing, 500 ml sample| diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors
Complete set of accessories for the Vivaflow® 50R|200 with a 115 V pump
Comprises: Pump (115 V), Easy Load pump head (size 16), tubing, 500 ml sample| diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors
Flow restrictor set (2 x 0.4, 0.6, 0.8 mm)
Flow restrictor set (2 x 0.4, 0.6, 0.8 mm) to be used with Vivaflow® systems
Pressure Indicator (1-3 bar)
Pressure indicator (1-3 bar) for Vivaflow® units
Flow restrictor 0.6 mm (6 pieces)
Flow restrictors 6 x 0.6 pink to be used with the Vivaflow® systems
500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.
Tygon Size 15 pump tubing for Vivaflow® 200
Size 15 pvc pump tubing and Luer fittings (3 m,4.8 x 2.6 mm)
Tygon Size 16 pump tubing for Vivaflow
Size 16 PVC pump tubing (3 m, 3.2 x 1.6 mm)