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Vivacon 2-PCR Grade, 30,000 MWCO Hydrosart, 25pc

100 µl - 2 ml
Vivacon® 2 are centrifugal units for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® ultrafiltration membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.
Vivacon® 2-PCR Grade units are treated with ethylene oxide (ETO) in a validated process to deactivate all traces of DNA that might interfere with subsequent amplification procedures.

Item no.:  VN02H21ETO

Availability (ex works):  On Request

Vivacon® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 2 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.

  • Concentration of up to 2 ml genomic DNA or peptide samples
  • Low binding material
  • Reverse spin possibility
  • MWCO printed on device
  • Complete and highly reproducible concentrate recovery
  • Selected cut offs available sterile and DNA free* for e.g. forensic applications
  • * qPCR assay over 55 cycles with a verified limit of detection < 2 pg

Performance Characteristics for DNA

Start volume 2 ml, sample concentration 50 ng/ml.

 Sample size (bp)Time to concentrate up
to 30x [min.] at 20°C
Concentrate
recovery %
g-force (xg)
2,000 MWCO10120 min.92 %7,500
10,000 MWCO3060 min.94 %5,000
30,000 MWCO5060 min.95 %2,500
50,000 MWCO30045 min.96 %2,500
100,000 MWCO60030 min.93 %2,500
125,000 MWCO65030 min.88 %2,500
125,000 MWCO90030 min.89 %2,500

Performance Characteristics for Proteins

Start volume 2 ml, sample and concentration of proteins as specified in table.
  Sample Time to concentrate up
to 30x [min.] at 20°C
Concentrate
recovery %
g-force (xg)
2,000 MWCO 0.25 mg/ml
cytochrome c
120 min. 95 % 7,500
10,000 MWCO 0.25 mg/ml
cytochrome c
90 min. 96 % 5,000
30,000 MWCO 1.0 mg/ml BSA 40 min. 96 % 5,000
50,000 MWCO 1.0 mg/ml BSA 30 min. 94 % 5,000
100,000 MWCO 1.0 mg/ml
bovine IgG
30 min. 92 % 5,000
125,000 MWCO 1.0 mg/ml
bovine IgG
27 min. 81 % 5,000
Start volume 2 ml, sample and concentration of proteins as specified in table.
  Sample Time to concentrate up
to 30x [min.] at 20°C
Concentrate
recovery %
g-force (xg)
2,000 MWCO 0.25 mg/ml
cytochrome c
120 min. 95 % 7,500
10,000 MWCO 0.25 mg/ml
cytochrome c
90 min. 96 % 5,000
30,000 MWCO 1.0 mg/ml BSA 40 min. 96 % 5,000
50,000 MWCO 1.0 mg/ml BSA 30 min. 94 % 5,000
100,000 MWCO 1.0 mg/ml
bovine IgG
30 min. 92 % 5,000
125,000 MWCO 1.0 mg/ml
bovine IgG
27 min. 81 % 5,000

Brochure

Ultrafiltration Family
en 1.6 MB
宣传手册:实验室过滤产品精简目录
zh 19.0 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB

Datasheet

Vivacon® 500 and Vivacon® 2 — For safe DNA and protein concentration and rebuffering from 500 μl to 2 ml starting volumes.
en 741.9 kB

User Manual

Vivacon® 2 — For in vitro use only
en 4.0 MB

Applications

  • Application
    • Protein Concentration
    • DNA Concentration
  • Area of Application
    • Buffer Exchange
    • Forensic Sample Prep
    • Protein/DNA Concentration
  • Market
    Forensic

Certificates

  • Certification
    None

Features

  • PCR Grade
    Yes

Materials of Construction

  • Concentrator Body/Sleeve
    Polycarbonate (PC)
  • Concentrator Cap
    Polypropylene (PP)
  • Filtrate Vessel
    Polypropylene (PP)
  • Membrane
    Hydrosart<sup>®</sup>
  • Membrane Material
    Hydrosart®

Physicochemical Information

  • MWCO
    30,000 Daltons

Product Information

  • Device Type
    Centrifugal Ultrafiltration Devices
  • Pack Size
    25
  • Primary Product Type
    Ultrafiltration Devices
  • Sample Type
    DNA

Volumes

  • Dead Stop Volume
    55 μl
  • Volume Range
    0.1 – 2 mL

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