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Sartobind® IDA A4 Sheet

Sartobind IDA Membrane Adsorbers are macroporous, cellulose-based membranes for binding of His-tagged proteins at high flow rates.

Item no.:  94IDA-42-001

Availability:  On Request

Sartobind® Membrane Adsorbers are made from regenerated cellulose and have been stabilized for high chemical stability. They are derivatized with conventional chelating functional groups to a metal chelate adsorber for protein and peptide purification with high selectivity. The large pores minimize the size exclusion effect. Membranes can be run at much higher flow rates compared to conventional matrices.

  • Macroporous
  • High flow rate
  • Simple handling

User Manual

Sartobind® IDA Sheet
en 32.3 kB

Product properties

  • Dimensions
    21 cm × 29.7 cm
  • Membrane area
    624 cm²
  • Pore size
    3 - 5 µm
  • Typical dynamic binding capacity 10 %
    >3.6 mg/mL, >100 μg/cm²
  • Membrane Material
    Stabilised reinforced cellulose
  • Pack Size
    1

Technical attributes

  • pH Stability
    pH 1 – 12 (short term), pH 3 – 9 (long term)
  • Membrane Type
    Metal chelate membrane
  • Ligand density
    > 5 µeq/cm²
  • Membrane thickness
    275 µm
  • Flow rate at 1 bar
    > 30 mL/cm² × min
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference Protein
    Polyhistidine tagged protein
  • What you receive
    • 1 sheet
    • 1 manual
  • Reinforcement Material
    Polyester
  • Ligand
    Iminodiacetic acid
  • Recommended Chelating Ions
    Ni²+, Co²+, Cu²+ or Zn²+
  • Reusable
    Yes