96-Channel Ultra High Throughput Octet® System

  • Analyze up to 96 samples simultaneously - Perform your quantitation or binding experiments with unmatched speed. Quantitate a full  96-well plate of IgGs in two minutes.
  • Perform a 32 x 32 epitope binning campaign in less than eight hours  - Use in-tandem, sandwich, or pre-mix assay formats to perform epitope binning. Analyze samples directly from supernatants or lysates. Complete a 32 x 32 epitope binning assay in less than eight hours.
  • Streamline workflows with automation – Automation-ready and robot-compatible for microplate and biosensor tray loading for extending walk-away times and reducing operator input.

From IgG titer to epitope binning, the Octet® RH96 instrument offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules. 

Simultaneously reads 8, 16, 32, 48 or 96 wells

The Octet® RH96 instrument read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized throughput up to 96 samples.

Full plate off-rate ranking in minutes, not hours

The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly, even in crude samples using ready-to-use biosensors off the shelf.

96-well plate quantitation in as little as two minutes

IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for greater sample throughput.

32 x 32 epitope binning in less than eight hours

With the Octet® RH96 system, epitope binning can be completed in record time, where a 32 x 32 antibody matrix can be analyzed in less than eight hours. A powerful dedicated epitope binning analysis software enables easy visualization and analysis of large data sets.

Microfluidics-free Dip and Read format reduces assay time and maintenance cost

The Octet® RH96 system utilizes Dip-and-Read technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs

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Applications

ELISA Conversion

ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations.  Convert your ELISA assay into an Octet® assay.

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Epitope Binning

The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries. 

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protein contaminant diagram

Protein Contaminant Testing

Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits  provide biologics developers with a fast and easy-to-use assay for contaminant testing.

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Featured Publications

Octet® Systems in Ligand Binding Assays That Meet Compliance

Octet® systems enable analytical assessment of biologics in various stages of the development workflow beginning with discovery and early...

Read Application Note
Label-Free Detection Technologies: Key Considerations and Applications cover

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

Read eBook

Automating Octet Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of...

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Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data

Automation

Robot compatible microplate and biosensor tray loading

Orbital flow capacity

Static or 100–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM – 10 pM

Baseline noise (RMS)

<3 pm (8–16 biosensors); <8 pm (32–96 biosensors)

Baseline drift

< 0.1 nm/hour

Quantitation range

32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm

Quantitation precision

CV <10%

Weight

200 lbs (90.7 kg)

Dimensions (H x W x D)

30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well and 384-well, black, tilted bottom microplates

Molecular weight detection

>150 Da (8–16 biosensors), >5000 Da (32–96 biosensors)


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