High Throughput Platform for Biologics Development

From IgG titer to epitope binning, the Octet® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.

High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. The Octet® RH16 instrument also supports additional automation for extending walk-away times and reducing operator input.  Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.

  • Analyze up to 16 samples simultaneously
  • Streamline workflows with automation - Automation ready and robot compatible for microplate and biosensor tray loading.
  • GxP packages available - Available as complete packages with 21 CFR Part 11 software and IQOQ/PQ kits, protocols and services for regulatory compliance.
Supports 96-well and 384-well microplate formats

Arrange assay samples in 96- or 384-well plates depending on workflows and sample volumes

Two microplate locations to maximize throughput and improve workflow

Supports larger sample and reagent capacities increasing walk-away times

Microfluidics-free Dip and Read format reduces assay time and maintenance cost

The Octet® RH16 system utilizes Dip-and-Read™ technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs

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Applications

ELISA Conversion

ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations.  Convert your ELISA assay into an Octet® assay.

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Epitope Binning

The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries. 

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protein contaminant diagram

Protein Contaminant Testing

Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits  provide biologics developers with a fast and easy-to-use assay for contaminant testing.

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Featured Publications

Octet® Systems in Ligand Binding Assays That Meet Compliance

Octet® systems enable analytical assessment of biologics in various stages of the development workflow beginning with discovery and early...

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Accurate Measurements on the Octet Platform

Without Added Sample Purification Steps

Read App Guide
Label-Free Detection Technologies: Key Considerations and Applications cover

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

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Automating Octet Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of...

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Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered

Data presentation

Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts

Automation

Robot compatible microplate and biosensor tray loading

Orbital flow capacity

Static or 100–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM to 10 pM

Baseline noise (RMS)

≤ 4 pm (RMS)

Baseline drift

< 0.1 nm/hour

Quantitation range

8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm

Quantitation precision

CV <10%

Weight

150 lb (68.2 Kg)

Dimensions (H x W x D)

30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well and 384-well, black, tilted bottom microplates

Molecular weight detection

>150 Da (8–16 biosensors)


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