Automated Small and Large Molecule Characterization

The 8-channel Octet RED96e systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. An optional microplate evaporation cover that minimizes losses in sample volume is available with the Octet RED96e.

  • Analyze samples in crude matrix - The fluidics-free Dip and Read technology with samples in a plate format combined with ready-to-use biosensors allows for high flexibility in sample type.
  • Characterize small and large molecules - Quantitate up to 96 samples in 32 minutes and perform kinetic screens of up to 64 samples in 1.5 hrs.
  • GxP packages available - The Octet RED96e System has been developed to operate reliably in a regulated environment. Complete packages are available with 21 CFR Part 11 software and IQOQPQ kits, protocols and services for regulatory compliance. Learn more about comprehensive GxP Compliance Tools for the Octet system.
High-quality kinetic screening and affinity characterization

The Octet RED96e system monitors binding events in real time to calculate on rates, off rates, and affinity constants.

Versatility to detect a wide range of molecules

The high sensitivity Octet RED96e System detects a diverse range of biomolecules from small molecules to proteins to mammalian cells.

Eight parallel, independent channels for maximum speed and flexibility

Each channel is independent of the others allowing for analysis of different sample types simultaneously. Analysis can be done using a single channel or up to eight channels.

Perfectly suited to operate in GxP-regulated environments

Optional 21 CFR Part 11 software for Octet RED96e available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.

Sample plate cooling for temperature sensitive proteins

The Octet RED96e operates with a wide range in temperature (15–40°C). An optional microplate evaporation cover minimizes sample loss enabling up to 12 hours of unattended run time.

Fluidics-free Dip and Read™ format reduces assay time and maintenance cost

The Octet provides faster time to results and process economy when compared to other technologies due to this format.

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Applications

Kinetic and Affinity Characterization

Octet instruments accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.

Protein Quantitation in Bioprocessing

Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA.

Quality Control (QC) and Manufacturing

The ease of use and simplicity in design together with a comprehensive tool set for compliance makes the Octet RED96e system an ideal analytical instrument in regulated quality control (QC) labs. The Octet is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration and impurity analysis in both upstream and downstream processes.

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Featured Publications

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

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Octet Kinetics Assay Method Development Guideline

Aimed at developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by the USP 1032...

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Writing Grant Proposals for Biomolecular Interactions Research

Grant writing can be a grind. You know how and why the funding is needed, but communicating that successfully sometimes seems like an art.

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Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

180–220 µL/well
Nondestructive testing, easily recovered

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates
Epitope binning and cross-blocking matrices and trace overlays
Tabulated kinetic or concentration data

Automation

Up to 8 assays in parallel; up to 96 assays per 96-well plate

Orbital flow capacity

Static or 100–1500 rpm

Analysis temperature

Analysis temp range: 15–40°C, 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM – 10 pM

Baseline noise (RMS)

<3 pm

Baseline drift

< 0.12 nm/hour

Quantitation range

0.5 µg/mL to 2000 µg/mL at 200 rpm,
0.05 µg/mL to 100 µg/mL at 1000 rpm

Quantitation precision

CV <10%

Weight

63 lb (28.6 Kg)

Dimensions (H x W x D)

18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well, black, flat-bottom microplate

Molecular weight detection

>150 Da


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