Automated Small and Large Molecule Characterization
The 8-channel Octet RED96e systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. An optional microplate evaporation cover that minimizes losses in sample volume is available with the Octet RED96e.
- Analyze samples in crude matrix - The fluidics-free Dip and Read technology with samples in a plate format combined with ready-to-use biosensors allows for high flexibility in sample type.
- Characterize small and large molecules - Quantitate up to 96 samples in 32 minutes and perform kinetic screens of up to 64 samples in 1.5 hrs.
- GxP packages available - The Octet RED96e System has been developed to operate reliably in a regulated environment. Complete packages are available with 21 CFR Part 11 software and IQOQPQ kits, protocols and services for regulatory compliance. Learn more about comprehensive GxP Compliance Tools for the Octet system.
The Octet RED96e system monitors binding events in real time to calculate on rates, off rates, and affinity constants.
The high sensitivity Octet RED96e System detects a diverse range of biomolecules from small molecules to proteins to mammalian cells.
Each channel is independent of the others allowing for analysis of different sample types simultaneously. Analysis can be done using a single channel or up to eight channels.
Optional 21 CFR Part 11 software for Octet RED96e available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.
The Octet RED96e operates with a wide range in temperature (15–40°C). An optional microplate evaporation cover minimizes sample loss enabling up to 12 hours of unattended run time.
The Octet provides faster time to results and process economy when compared to other technologies due to this format.
Applications
Kinetic and Affinity Characterization
Octet instruments accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.
Protein Quantitation in Bioprocessing
Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA.
Quality Control (QC) and Manufacturing
The ease of use and simplicity in design together with a comprehensive tool set for compliance makes the Octet RED96e system an ideal analytical instrument in regulated quality control (QC) labs. The Octet is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration and impurity analysis in both upstream and downstream processes.
Featured Publications
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 180–220 µL/well Nondestructive testing, easily recovered |
Data presentation | Sensorgrams displaying kinetic traces or concentration binding rates |
Automation | Up to 8 assays in parallel; up to 96 assays per 96-well plate |
Orbital flow capacity | Static or 100–1500 rpm |
Analysis temperature | Analysis temp range: 15–40°C, 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM – 10 pM |
Baseline noise (RMS) | <3 pm |
Baseline drift | < 0.12 nm/hour |
Quantitation range | 0.5 µg/mL to 2000 µg/mL at 200 rpm, |
Quantitation precision | CV <10% |
Weight | 63 lb (28.6 Kg) |
Dimensions (H x W x D) | 18.6 in (H) x 17 in (D) x 20.8 in (W) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well, black, flat-bottom microplate |
Molecular weight detection | >150 Da |
- Brochure: Octet Systems PDF | 5.2 MB
- Selection Guide: Octet Biosensors PDF | 299.7 KB
- Datasheet: Octet CFR Software Validation Package PDF | 261.1 KB
- Datasheet: Octet Data Analysis HT Software Version 12 PDF | 649.8 KB
- Datasheet: Octet Software Comparisons PDF | 183.4 KB
- Datasheet: Octet BLI Discovery and Octet Analysis Studio Software Version 10.0+ PDF | 1.2 MB
- Datasheet: Sidekick Offline Biosensor Immobilization Station PDF | 222.7 KB
- Datasheet: Octet Red96e Systems PDF | 987.5 KB
- Datasheet: Octet System Qualification Products And Services PDF | 83.9 KB
- Manual: Microplate Evaporation Covers PDF | 673.1 KB
- Application Note: Enhancing Efficiency And Economics In Process Development And Manufacturing Of Biotherapeutics PDF | 543.3 KB