High Throughput Platform for Biologics Development
From IgG titer to epitope binning, the Octet RED384 system offers label-free molecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
The high detection sensitivity and throughput of the Octet RED384 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding matrices. Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.
- Analyze up to 16 samples simultaneously
- Streamline workflows with automation - Automation ready and robot compatible for microplate and biosensor tray loading.
- GxP packages available - Available as complete packages with 21 CFR Part 11 software and IQOQPQ kits, protocols and services for regulatory compliance.
Use up to 384 samples in one 384-well microplate for kinetics analysis
The Octet RED384 system utilizes Dip and Read™ technology with the read head dipping into samples in a plate format. With no fluidics involved, the Octet 384 system has more uptime and a lower cost of maintenance.
Applications
ELISA Conversion
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet systems are designed to overcome those limitations. Convert your ELISA assay into an Octet assay.
Epitope Binning
Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a rapid and easy to use technique for contaminant testing.
Featured Publications
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered |
Data presentation | Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts |
Automation | Robot compatible microplate and biosensor tray loading |
Orbital flow capacity | Static or 100–1500 rpm |
Analysis temperature | Ambient + 4°C to 40°C, in 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM to 10 pM |
Baseline noise (RMS) | ≤ 4 pm (RMS) |
Baseline drift | < 0.1 nm/hour |
Quantitation range | 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm |
Quantitation precision | CV <10% |
Weight | 150 lb (68.2 Kg) |
Dimensions (H x W x D) | 30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well and 384-well, black, tilted bottom microplates |
Molecular weight detection | >150 Da (8–16 biosensors) |
- Brochure: Octet Systems PDF | 5.2 MB
- Datasheet: 384 Well Tilted Bottom Microplate PDF | 189.0 KB
- Datasheet: Octet CFR Software Validation Package PDF | 261.1 KB
- Datasheet: Octet Data Analysis HT Software Version 12 PDF | 649.8 KB
- Datasheet: Octet Red384 Systems PDF | 1.0 MB
- Datasheet: Octet Software Comparisons PDF | 183.4 KB
- Datasheet: Octet BLI Discovery and Octet Analysis Studio Software Version 10.0+ PDF | 1.2 MB
- Datasheet: Octet System Qualification Products And Services PDF | 83.9 KB
- Datasheet: Sidekick Offline Biosensor Immobilization Station PDF | 222.7 KB
- Selection Guide: Octet Biosensors PDF | 299.7 KB
- Application Note: Enhancing Efficiency And Economics In Process Development And Manufacturing Of Biotherapeutics PDF | 543.3 KB
- Application Note: Converting ELISA Assay Into Octet Quantitation Assay PDF | 304.4 KB