High Performance, Research-Friendly Kinetics Characterization
The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data.
Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.
The Octet K2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates.
- Sensitivity for small molecule analysis - The Octet K2 System enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.
- Flexible second channel for use as a reference or more samples - Unique 2-channel design has dedicated spectrometers for simultaneous referencing or added capacity.
- Simple setup for protein and antibody characterization - Template driven interface allows new users to get started quickly for protein and antibody characterization.
The Octet K2 System monitors binding events in real time to calculate on rates, off rate, and dissociation constants. The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference. Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. With predefined templates to follow in Octet data acquisition software, the system streamlines setup prior to running an assay and minimizes training needs. The software then gives users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.
Utilizing ForteBio's one-step Dip and Read™ assays, the Octet K2 System directly measures the presence of specific proteins and other molecules in solution. Accurate and reproducible concentrations can be determined in as little as two minutes per sample.
The Octet K2 System provides the ability to rerack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.
Applications
Large Molecule Characterization
With pre-defined templates to follow in Octet Data Acquisition software, the Octet K2 system streamlines setup prior to running an assay and minimizes training needs. Octet Data Analysis software then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface
Small Molecule Characterization
The Octet K2 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and dissociation constants (KD). The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference.
Featured Publications
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 180–220 µL/well Nondestructive testing, easily recovered |
Data presentation | Sensorgrams displaying kinetic traces or concentration binding rates |
Automation | Up to 2 assays in parallel |
Orbital flow capacity | Static or 400–1500 rpm |
Analysis temperature | Ambient + 4°C to 40°C, in 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM – 10 pM |
Baseline noise (RMS) | <3.5 pm |
Baseline drift | < 0.1 nm/hour |
Quantitation range | 0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) |
Quantitation precision | CV <10% |
Weight | 58 lb (26.3 Kg) |
Dimensions (H x W x D) | 18.6 in (H) x 17 in (D) x 20.8 in (W) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well, black, flat-bottom microplate |
Molecular weight detection | >150 Da |
- Brochure: Octet Systems PDF | 5.2 MB
- Datasheet: Octet Software Comparisons PDF | 183.4 KB
- Datasheet: Octet BLI Discovery and Octet Analysis Studio Software Version 10.0+ PDF | 1.2 MB
- Datasheet: Octet System Qualification Products And Services PDF | 83.9 KB
- Datasheet: Octet Data Analysis HT Software Version 12 PDF | 649.8 KB
- Selection Guide: Octet Biosensors PDF | 299.7 KB