Octet^® K2 2-Channel System Has Been Discontinued.

Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates
Epitope binning and cross-blocking matrices and trace overlays
Tabulated kinetic or concentration data

Automation

Up to 2 assays in parallel

Orbital flow capacity

Static or 400–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (k_a)

10¹ to 10⁷ M^-1 s-¹

Dissociation rate constant (k_d)

10^-6 to 10^-1 s^-1

Affinity constant (K_D)

1 mM – 10 pM

Baseline noise (RMS)

<3.5 pm

Baseline drift

< 0.1 nm/hour

Quantitation range

0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) 
Protein - and assay-dependent

Quantitation precision

CV <10%

Weight

58 lb (26.3 Kg)

Dimensions (H x W x D)

18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well, black, flat-bottom microplate

Molecular weight detection

>150 Da