2-Channel Entry-Level Octet System: Octet K2

Request a Quote

High Performance, Research-Friendly Kinetics Characterization

The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data.

Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.

The Octet K2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates.

  • Sensitivity for small molecule analysis - The Octet K2 System enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.
  • Flexible second channel for use as a reference or more samples - Unique 2-channel design has dedicated spectrometers for simultaneous referencing or added capacity.
  • Simple setup for protein and antibody characterization - Template driven interface allows new users to get started quickly for protein and antibody characterization.
Octet K2
Kinetic Analysis

The Octet K2 System monitors binding events in real time to calculate on rates, off rate, and dissociation constants. The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference. Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. With predefined templates to follow in Octet data acquisition software, the system streamlines setup prior to running an assay and minimizes training needs. The software then gives users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.

Quantitative measurements

Utilizing ForteBio's one-step Dip and Read™ assays, the Octet K2 System directly measures the presence of specific proteins and other molecules in solution. Accurate and reproducible concentrations can be determined in as little as two minutes per sample.

Biosensor Re-racking

The Octet K2 System provides the ability to rerack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.

Request a Quote

Applications

Large Molecule Characterization

With pre-defined templates to follow in Octet Data Acquisition software, the Octet K2 system streamlines setup prior to running an assay and minimizes training needs. Octet Data Analysis software then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface

Small Molecule Characterization

The Octet K2 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and dissociation constants (KD). The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference.

Read Application Note

Contact a Specialist

Featured Publications

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

Read eBook

Writing Grant Proposals for Biomolecular Interactions Research

Grant writing can be a grind. You know how and why the funding is needed, but communicating that successfully sometimes seems like an art.

Read White Paper

Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

180–220 µL/well
Nondestructive testing, easily recovered

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates
Epitope binning and cross-blocking matrices and trace overlays
Tabulated kinetic or concentration data

Automation

Up to 2 assays in parallel

Orbital flow capacity

Static or 400–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM – 10 pM

Baseline noise (RMS)

<3.5 pm

Baseline drift

< 0.1 nm/hour

Quantitation range

0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) 
Protein - and assay-dependent

Quantitation precision

CV <10%

Weight

58 lb (26.3 Kg)

Dimensions (H x W x D)

18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well, black, flat-bottom microplate

Molecular weight detection

>150 Da


Request More Information

Related Products

Octet HTX

96-Channel Ultra High Throughput Octet System: Octet HTX

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

Explore More
Octet RED384

16-Channel High Throughput Octet System: Octet RED384

The Octet RED384 16-channel instrument provides analytical performance similar to the 8- channel Octet RED96 system, but with higher levels of...

Explore More
Octet RED96e

8-Channel Octet System: Octet RED96e

The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours.

Explore More
Octet QKe

8-Channel Octet System: Octet QKe

Unmatched versatility for discovery, development, and quality control

Explore More
BLItz System

Personal assay BLItz System

Label-free assays in a drop

Explore More
Octet Sidekick

Sidekick™ Offline Biosensor Immobilization Station

The Sidekick station enables simultaneous and uniform reagent loading onto all 96 biosensors in a biosensor tray.

Explore More