96-Channel Ultra High Throughput Octet® RH96
- Analyze up to 96 samples simultaneously - Perform your quantitation or binding experiments with unmatched speed. Quantitate a full 96-well plate of IgGs in two minutes.
- Perform a 32 x 32 epitope binning campaign in less than eight hours - Use in-tandem, sandwich, or pre-mix assay formats to perform epitope binning. Analyze samples directly from supernatants or lysates. Complete a 32 x 32 epitope binning assay in less than eight hours.
- Streamline workflows with automation – Automation-ready and robot-compatible for microplate and biosensor tray loading for extending walk-away times and reducing operator input.
From IgG titer to epitope binning, the Octet® RH96 instrument offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
Discover the Octet® RH16 & RH96 Systems
Features of the Octet® RH96
The Octet® RH96 instrument read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized throughput up to 96 samples.
The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly, even in crude samples using ready-to-use biosensors off the shelf.
IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for greater sample throughput.
With the Octet® RH96 system, epitope binning can be completed in record time, where a 32 x 32 antibody matrix can be analyzed in less than eight hours. A powerful dedicated epitope binning analysis software enables easy visualization and analysis of large data sets.
The Octet® RH96 system utilizes Dip and Read technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs
Applications for the Octet® RH96
ELISA Conversion
ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations. Convert your ELISA assay into an Octet® assay.
Epitope Binning
The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries.
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing.
Educational Resources
Product Resources
Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics
PDF | 372.5 KB
Technology | Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Sample volume | 40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered |
Data presentation | Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data |
Automation | Robot compatible microplate and biosensor tray loading |
Orbital flow capacity | Static or 100–1500 rpm |
Analysis temperature | Ambient + 4°C to 40°C, in 1°C increments |
Association rate constant (ka) | 101 to 107 M-1 s-1 |
Dissociation rate constant (kd) | 10-6 to 10-1 s-1 |
Affinity constant (KD) | 1 mM – 10 pM |
Baseline noise (RMS) | <3 pm (8–16 biosensors); <8 pm (32–96 biosensors) |
Baseline drift | < 0.1 nm/hour |
Quantitation range | 32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm |
Quantitation precision | CV <10% |
Weight | 200 lbs (90.7 kg) |
Dimensions (H x W x D) | 30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm) |
Sample type | Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates |
Sample plate | Standard, 96-well and 384-well, black, tilted bottom microplates |
Molecular weight detection | >150 Da (8–16 biosensors), >5000 Da (32–96 biosensors) |
