Octet® AAVX Biosensors
Rapid Quantitation of AAV Capsids for Various Serotypes for Bioprocess Development and Quality Control in Gene Therapy
- Rapid, direct, and label-free quantitation of AAV capsids in crude and purified samples
- Specificity to various AAV serotypes, including AAV1 – AAV9 and AAVrh10
- Direct quantitation of AAV capsids in the dynamic range of 8.5E8 – 1.0E13 vp/mL
- Efficient and cost-effective regeneration for biosensor reuse up to 10 - 20 times
- Good correlation of the results with ELISA
Adeno-associated virus (AAV), with low immunogenicity and toxicity, has become one of the most promising viral vectors used for delivery of genetic material in gene therapy. AAV vector development and production at industrial scale requires an efficient method for accurate quantitation of viral capsids in both upstream and downstream bioprocessing. Octet® Bio-Layer Interferometry (BLI) systems in combination with the Octet® AAVX Biosensors offer a rapid, label-free, and high-throughput method for quantification of AAV capsids at various stages in bioprocessing.
Octet® AAVX Biosensors are pre-immobilize with Thermo ScientificTM CaptureSelectTM Biotin Anti-AAVX Conjugate, from Thermo Fisher Scientific Inc, that binds with high affinity and specificity to various native and recombinant AAV serotypes, including AAV1 to AAV9 and AAVrh10. Due to its high specificity the AAVX biosensor can be used to quantitate AAV capsids in both purified and crude cell culture samples with titer in the range of 8.5E8 – 1.0E13 vp/mL for most serotypes.
AAV titer can be measured on the Octet® platform in as little as 15 minutes, providing an efficient alternative to time-consuming and laborious techniques such as ELISA and HPLC. Finally, the Octet® AAVX Biosensors can be efficiently regenerated up to 20 times, which makes them an extremely useful and cost-effective solution for high-throughput AAV capsid titer measurement in process development and QC.
Generalized Workflow for AAV Vector Production and Technology Solutions Offered by Sartorius to Support the AAV Production Workflow
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*CaptureSelect is a trademark of Thermo Fisher Scientific