Octet® SAX2 Biosensors

For High Precision and Reproducible Kinetic Characterization and Custom Quantitation

  • QC-tested to limit biotinylated ligand loading variance, providing lot-to-lot biosensor consistency
  • Minimal lot-to-lot biosensor variance enables confident detection of variance from samples
  • Rapid and stable capture of biotinylated molecules

Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors enable quick and easy modification and customization of the biosensor with any biotin-tagged molecule for quantitative and kinetic measurements. The Octet® SAX2 biosensors are high precision streptavidin biosensors that were specifically developed and qualified for applications in downstream drug discovery and regulated environments that have stringent assay precision requirements.

Octet® SAX2 biosensors are QC-tested at Sartorius to meet our precision-controlled lot-to-lot coefficient of variation (CV) specification of <20% for binding of biotin-tagged molecule onto the biosensor. Within lot, Octet® SAX2 biosensors are QC-tested to meet CV of <4%.

Range of Applications

Octet® SAX2 biosensors are designed and optimized for applications that require minimal variation from consumables, to allow for accurate measurements of variance from samples. These biosensors are best suited for:

  • Product release QC
  • Activity assays in manufacturing
  • Highly precise titer determination

Octet® SAX2 Biosensors

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Featured Resources

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Application Note

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet® SAX Biosensors

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Application Note

MAb Quantitation Protein A HPLC vs Protein A Bio-Layer Interferometry

Rapid, accurate and cost-effective quantitation of monoclonal antibodies (MAbs) is essential for bioprocessing.

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Octet® High Precision Streptavidin 2.0 (SAX2) Biosensors
Technical Note

High Precision Streptavidin 2.0 (SAX2) Biosensors

Streptavidin is often deployed as the molecule of choice for covalent linking to solid surfaces for ligand binding assays applications.

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Enhanced Efficiency in Lot Release and In-Process Testing of Biologics cover
White Paper

Enhanced Productivity and Labor Efficiency

In Lot Release and In-Process Testing of Biologics in GxP Laboratories

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Additional Resources

Technical Note

Analysis of FLAG Proteins on the Octet®️ BLI Platform Using SAX or SAX2 Biosensors

PDF | 901.4 KB
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Datasheet

Octet® High Precision Streptavidin 2.0 (SAX2) Biosensors

PDF | 2.5 MB
selection guide
Flyer

Octet® BLI Biosensor Selection Guide

PDF | 404.8 KB
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Application Note

Fast Quantitation of Proteins and Antibodies Using the Octet® N1 System

PDF | 350.2 KB

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