First Virus Retentive Filter for Cell Culture Media
Virus-retentive filtration is a highly efficient method for viral risk mitigation of cell culture media. This filter targets both non-enveloped viruses, such as MVM and vesivirus, and large enveloped viruses, such as MuLV. Virosart® Media is especially designed for virus filtration of chemically defined cell culture media.
Virus Risk Mitigation in Media Preparation
Multiple bioreactor contaminations reported over the past several years have been caused by small, non-enveloped viruses like MVM and vesivirus derived from raw materials.
A new strategy is the removal of viruses before the bioreactor already after media preparation to prevent potential contaminations of the bioreactor. Virus filtration is a proven and well established technology within the market to remove all kind of viruses (> 20 nm range).
Our Solution: Virosart® Media
Virosart® Media is a virus retentive filter specially developed for upstream applications. The newly developed media filter overcomes today’s bottlenecks of virus filters originally developed for downstream applications and provides a cost effective solution in upstream with highest filter capacity for cell culture media.
Viral risk mitigation in media preparation of:
- Chemically defined cell culture media
- Addition of media supplement such as CaCl2
- Powder as well as liquid media
Highest safety for the bioreactor
New high-performance PES membranes
|Sterile delivery due to gamma irradiatable capsule design|
The performance of the newly developed virus retentive filter for risk mitigation within cell culture media was evaluated for different commercially available media.
The Virosart® Media shows high retention for all tested organisms.
|PP7||Proven bacteriophage model for parvoviruses||≥ 4|
|MVM||Model virus for small non-enveloped virus||≥ 4|
|MuLV||Model virus for large enveloped viruses|
In total >15 different commercially available cell culture media were tested with the Virosart® Media.