Virus Retention for Cell Culture Media

Virus-retentive filtration is a highly efficient method for viral risk mitigation of cell culture media. This filter targets both non-enveloped viruses, such as MVM and vesivirus, and large enveloped viruses, such as MuLV. Virosart® Media is especially designed for virus filtration of chemically defined cell culture media.

Challenges in Virus Risk Mitigation for Media Preparation

Multiple bioreactor contaminations reported over the past several years have been caused by small, non-enveloped viruses like MVM and vesivirus derived from raw materials.

A New Virus Risk Mitigation Strategy

A new strategy is the removal of viruses before the bioreactor already after media preparation to prevent potential contaminations of the bioreactor. Virus filtration is a proven and well established technology within the market to remove all kind of viruses (> 20 nm range).

Our Solution: Virosart® Media

Virosart® Media is a virus retentive filter specially developed for upstream applications. The newly developed media filter overcomes today’s bottlenecks of virus filters originally developed for downstream applications and provides a cost effective solution in upstream with highest filter capacity for cell culture media.

Virus risk mitigation in media preparation of:

  • Chemically defined cell culture media
  • Addition of media supplement such as CaCl2
  • Powder as well as liquid media

Highest safety for the bioreactor:

  • ≥ 4 LRV for small non-enveloped viruses
  • ≥ 6 LRV for large enveloped viruses

New high-performance PES membranes:

  • Economical solution for upstream virus media filtration

  • Highest capacities and flow rates

  • No impact on cell culture performance

Sterile delivery due to gamma irradiatable capsule design:

  • Ready to use
  • Easy implementation into single-use processes

Technical Proof

Retentive Capacities

OrganismDescriptionLRV

MVM

Model virus for small non-enveloped virus  

≥ 4

MuLV

Model virus for large enveloped viruses    

≥ 6

Virosart® Media shows high retention for all tested organisms. The reliable performance of the media virus retentive filter helps to mitigate risk and enhance speed from process to commercial scale.

Performance

In total >15 different commercially available cell culture media were tested with the Virosart® Media. 

Product Overview

Order Information

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Virus Risk Mitigation in Cell Culture Media

PDF | 527.9 KB

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Filterability Performance

PDF | 1.7 MB

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Retention Characteristics

PDF | 577.6 KB

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Influence on Media Components on Filtration

PDF | 345.7 KB

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Cell Growth

PDF | 1002.2 KB

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Lot to Lot Performance Consistency

PDF | 850.9 KB

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Virus Clearance Under Challenging Conditions

PDF | 225.5 KB

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Filtration of Calcium Chloride

PDF | 1.4 MB

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Virosart® Media

PDF | 617.1 KB

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New Solutions for Virus Risk Mitigation in Cell Culture Media

PDF | 832.2 KB

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Case Study: Virus Risk Mitigation in Cell Culture Media

PDF | 655.5 KB

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Contamination Risk Mitigation in Cell Culture Media Preparation

PDF | 550.0 KB

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How to Use Virosart® Media

PDF | 964.7 KB

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