Sartobind® Rapid A Membrane for mAb Capture

Replace Columns With A Scalable, Ready-To-Use & Disposable Alternative

Resin-based packed bed chromatography for mAb capture hampers downstream processing with low productivity, laborious cleaning requirements, and high costs.

Our Sartobind® Rapid A Membrane is a game-changing, disposable plug-and-play alternative that uses a one-batch, one-membrane approach. Compared to resin chromatography, Sartobind® Rapid A membranes deliver up to 14x more productivity during capture in rapid cycling mode.

Additionally, unlike resins, membranes do not need to be packed, significantly reducing non-value-added steps, reducing hands-on time by 80% compared to resin-based chromatography in batch mode.

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Webinar | Imagine Seamless Scale up to Rapid Cycling

Learn how chromatography membranes offer advantages over protein A resins.

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Sartobind® Rapid A - Plug and Play mAb Capture 

Traditional mAb capture methods are inefficient and costly. Sartobind® Rapid A from Sartorius changes the game with a one-batch, one-membrane alternative that cuts time, risks, and costs associated with resin-based batch operations.

Eliminate Costly Column Activities

No more column handling or risky consumable reuse — Sartobind® Rapid A removes the need for resin.

Cut Cost of Bulk Resin and Underutilization

Sartobind® Rapid A is fully utilized and cuts costs using less chromatographic material.

Accelerate Speed to Clinic

Rapid cycling and high binding capacity boost productivity up to 10x. Perform lifetime studies in one day.

Fully Scalable

Scale up based on binding capacity with constant residence time, productivity, and operating pressures.

Cut Risk of Bioburden Linked to Columns

Using a new membrane for each batch eliminates the risk of bioburden.

Scale Up Faster With Zero Columns

Adopt a Fully Proven Scalable Solution From PD to Commercial Scale

Sartobind® Rapid A is the first portfolio to cover PD, clinical and commercial scales. A constant bed height over different device sizes allows easy scale-up based on binding capacity, preserving productivity, flow rates, and quality.

  • Scale up seamlessly
  • Simplify linear scalability with modular cassette format
  • Benefit from short cycle times, high productivity, and operating pressures <0.2 MPa

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Scale Up Easily With the Sartobind® Rapid A Portfolio

Sartobind® Rapid A devices include nano- and mini-sized solutions for benchtop systems, larger capsules for pilot testing, and modular cassettes for commercial manufacturing. The family of products eases the gradual expansion of rapid cycle chromatography across your operations.

  • Ligand: Protein A
  • Bed height: 4 mm

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Attributes

Sartobind® Rapid A 96-Well Plate​

Sartobind® Rapid A NanoSartobind® Rapid A Mini

Sartobind® Rapid A 75 mL

Sartobind® Rapid A 200 mL

Sartobind® Rapid A Cassette

Scale of Operation

Candidate screening and process development

Process development

Process development

Clinical scale

Clinical scale

Late clinical to commercial scale

Membrane Volume

19 µL/well

1.2 mL

10 mL

75 mL

200 mL

800 mL

Connector

 

Female luer

Female luer; sanitary ¾”,
25 mm

Sanitary ¾”,
25 mm

Sanitary 1 ½”, 50.5 mm

Via manifold accessory: sanitary 1 ½”,
50.5 mm

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Save Time, Cut Costs and Reduce Risk With Sartobind® Rapid A

Rapid Cycling Yields Higher Productivity at Lower Cost With Less Risk

Sartobind® Rapid A’s one-batch, one-membrane approach minimizes bioburden risks, cuts unproductive activities, and saves on developing therapeutics by removing the high cost of bulk resin. Sartobind® Rapid A represents the next generation of mAb capture.

  • Cut 80% of non-productive column-use activities
  • Save up to 30% on process costs in clinical scale production
  • Minimize cross-contamination risk

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Traditional Batch vs. Rapid Cycling Chromatography

Break Through DSP Bottlenecks With Sartobind® Rapid A

Process intensification efforts on the upstream side have contributed to bottlenecks downstream. Typical batch approaches to mAb capture can lengthen process times. To break through this bottleneck in downstream processing, Sartorius developed a new technology. Rapid cycling chromatography with Sartobind® Rapid A improves productivity to accelerate speed to clinic.

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Rapid Cycling Chromatography

Rapid cycling chromatography purifies a batch over a large number of very short cycles. To make this possible, a new matrix was developed that allows comparable binding capacity at shorter residence time (higher flow rates).

  • One small device for mAb capture
  • One batch is purified over a large number of cycles
  • 10-15 minute cycles
  • Typical productivity of the mAb capture step: > 150 g/L*h
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Packed Bed Chromatography in Batch Approach

Packed bed chromatography in batch mode is often used to purify mAbs. One unit operation is completed at a time. A volume of mAbs is purified over a set number of cycles in a large column with a packed bed of protein A resin. The eluate from each batch is combined before virus inactivation.

  • 3-6 cycles per batch
  • Complete a single cycle in 2-4 hours
  • Protein A resin productivity: 10 – 20 g/L*h

Chromatography System Solutions Ideally Suited for Sartobind® Rapid A

Multi-Use Membrane Chromatography System: Resolute®️ MU RCC

Resolute®️ MU RCC is a dedicated membrane chromatography system that perfectly fits Sartobind®️ Rapid A 75 mL and 200 mL capsules.

Explore Resolute®️ RCC MU

Single-Use Batch Chromatography System: Resolute®️ Flowdrive SU

Resolute® Flowdrive SU with Sartobind® Rapid A supports single-use processes and fits 200 mL capsules and up to 3 cassettes.

Explore Resolute®️ Flowdrive SU

Learn More About Rapid A Chromatography

The Next Generation of Chromatography

Conventional chromatographic matrices include, among others, resins and membranes. Mass transport in resins relies on diffusion, while in membranes, it uses convection. Both have drawbacks. Mass diffuses slowly through resins, causing low productivity. Membrane structures force users to accept a tradeoff of inversely related production factors. However, a new generation of chromatographic materials combines the structure of resins with the convection of membranes. This ensures productivity, robustness, scalability, and easy cleaning.

Featured Assets

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Want to Remove the mAb Capture Bottleneck?

See first-hand how Sartobind® Rapid A delivers a tenfold increase in productivity with Sartorius’ new chromatography membrane. Learn more about the product’s scalability, from PD to commercial, and how to remove the mAb capture bottleneck in this webinar.

Watch Now

White Paper

Learn How to Benefit From a Thoughtful Process Development Strategy

PDF 1.7 MB
White Paper

Solve Your mAb Capture Scaling Challenges

PDF 1.3 MB
White Paper

Examine the Research Results | Sartobind® Rapid A for mAb Capture ...

PDF 1.5 MB

Science Snippets Blog

Cell Line Development
Apr 06, 2023 | 4 min read

Optimizing Cell Line Development With Automated mAb Sample Preparation

Cell line developers often handle 20-50 samples simultaneously. Streamlined sample preparation solutions can help eliminate the ensuing downstream bottlenecks.

Chromatography
Mar 29, 2023 | 4 min read

Cost-Effectivity Due to Improved Productivity in mAb Capture

It’s time to be practical about productivity in chromatography. Find out how to make your mAb capture more productive, and the additional benefits of switching from resins to membranes.

Chromatography
Oct 18, 2022 | 3 min read

Optimizing mAb Process Development for 10X Productivity

Demand on mAb process development requires a new and holistic approach. Thoughtful process development using new membrane technology is a game-changing strategy, with benefits hard to ignore.

Chromatography
Dec 16, 2021 | 8 min read

Resins, Monoliths, or Membranes. Which Chromatographic Method Should You Use? 

Which chromatography matrix is right for your application? Compare resin, monolith, and membrane formats to find the best fit.

Article

Learn how scalable mAb capture solution boosts productivity

Curious about research findings on Sartobind® Rapid A for mAb capture? Read the BPI article for productivity and scalability results.

Download

Frequently Asked Questions

Protein A chromatography is the most efficient stable affinity chromatography technique to concentrate and purify antibodies or Fc containing proteins in batch or continuous mode.

It is a proteinic ligand providing highly specific interaction with an antibody. It binds under mild conditions and elutes at lower pH enabling the removal of many product/process-related impurities.

It is a recombinant protein produced from mammalian cells (Chinese Hamster Ovary). It is secreted in the cell culture supernatant under perfusion or fed-batch conditions with titers up to 10 g/L.

It is a technique based on very high frequency of cycling of a quasi-flow rate independent chromatography consumable allowing much higher productivity and enabling a single-batch strategy.

Related Assets

White Paper

Re-Imagining Chromatography - Building Efficient, Connected Downstream Processes

PDF | 2.4 MB
Application Note

A New Way to Perform mAb Capture: Scaling Rapid Cycling Membrane Chromatography From Bench to Commercial Manufacturing

PDF | 492.4 KB
Application Note

Sartobind® Rapid A: High Binding Capacities at Short Residence Times

PDF | 763.4 KB
Application Note

Sartobind® Rapid A: Proven, Scalable New Membrane Platform for mAb Capture

PDF | 493.4 KB
Application Note

Sartobind® Rapid A: Comparable High Product Quality as Protein A Resins at High Productivity

PDF | 3.0 MB
Application Note

Sartobind® Rapid A:  Robust mAb Capture at High Productivity

PDF | 3.8 MB
Datasheet

Sartobind Rapid® A

PDF | 527.5 KB

Consult Our Experts

Consult Our Experts

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