Live-Cell Imaging and Analysis Instrument: Incucyte® SX5

With the Incucyte® SX5 Live-Cell Analysis System, your research can flourish in a label-free, non-perturbing environment. See more information in every sample and explore more applications with patent-pending optics. Leverage up to five total fluorescence channels (up to three simultaneously) for long term time-lapse experiments, including a long wavelength, low phototoxicity NIR channel. 

Key Features:

  • Our most advanced application offering, including neuronal activity and metabolism
  • Multi-user, multi-application support through remote network capability, unlimited free user licenses, independent vessel scheduling, and multi-objective scheduling.
  • A Green/Orange/NIR optical module included, with optional Green/Red and Metabolism optical modules
  • 4x, 10x, 20x objectives on an automated turret.
  • Support for 3 interchangeable vessel trays and over 700 vessels, compatible with flask, dishes, slides, and microplates - up to 6 microplates in parallel.

Enjoy walk-away convenience as images are automatically acquired and analyzed in a variety of formats within the stable environment of a tissue culture incubator. Insight, accuracy and reliability is available at every step in the process, from culture maintenance to final experimental assay.  Go where your research takes you with more colors, more insights, more possibilities!

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Explore the Incucyte® SX5

See what the SX5 has to offer in Live-Cell Imaging & Analysis

More Colors. More Insights. More Possibilities.

Protective Shield

Relevant Environment

Cells sit stationary in incubator's stable environment as optics move below to capture biology as it unfolds - no disruption of cell cultures

Product Hub

Remote, Networked Access

Access the instrument anytime, anywhere –  perfect for routine assay monitoring and restrictive laboratory conditions


Purpose-Built Software

User-friendly software for all scientists across variety of cell models - run complex assays and generate reliable, objective, publication-ready data

Idea in Mind

More Insight

Accommodate multiple users and applications with up to six microplates, two different fluorescent channels and three objectives


Browse Publications

See how your peers are accelerating their studies with the latest Incucyte® research, featuring over 7000 references in peer-reviewed publications

Explore Our Publications

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Learn how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data with a virtual demo

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Incucyte® SX5 Applications

Accelerate your next discovery with Incucyte®’s suite of live-cell applications. Gain real-time morphological and phenotypic insight for pathway and mechanistic studies by capturing time-dependent and cell-dependent treatment effects. 

From the moment cells are placed in culture, the learning opportunity begins. Whether you’re working with rare or expensive cell types (e.g., primary cells, stem-cell derived cultures, patient derived cells) or recombinant cell lines, Incucyte® live-cell applications enable you to quantitate and validate cell morphology and phenotype during all stages of cell maintenance, modification as well as perform analysis on a wide range of phenotypic cell-based assays.

Make informed decisions about your cultures, rapidly optimize and improve your workflows, plus study complex live-cell assays to fast track your next discovery! 

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Imaging Vessels

The Incucyte® Live-Cell Analysis Systems are compatible with a wider range of culture vessels – currently over 700 and growing! From 6-well to 384-well microtiter plates to standard tissue culture flasks, the Incucyte® can support your live-cell imaging and analysis needs.

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SX5 Specifications




Optical System

Fluorescence Channels

Up to 5

Optical Module 

Green/Orange/NIR (included)
Green/Red (available for purchase)
Metabolism (available for purchase)

Label-Free HD Phase Imaging


4x, 10x, 20x (included)

Objective Automation 

Automated Turret 

Multi-User Support

Remote Network Capability
Unlimited, Free user licenses
Independent Vessel Scheduling
Multi-Objective Scheduling


Incubator Size 

>200 L required

Incubation to 42°C

Software Module Compatibility

Incucyte® Cell-by-Cell 
Incucyte® Spheroid
Incucyte® Cell Migration 
Incucyte® Chemotaxis 
Incucyte® Neurotrack 
Incucyte® Neuronal Activity 
Incucyte® Angiogenesis 
Incucyte® AI Cell Health 
Incucyte® ATP Analysis

Capacity and Vesselware Compatibility 

Interchangeable Trays


Microplate Capacity 


Microplate Compatibility 

6, 12, 24, 48, 96, 384-well

Flask Compatibility 

T-25, T-50, T-75, T-100, T-175, T-225

Other Labware Compatibility

35mm, 60mm, 100mm, 150mm dishes and chamber slides

Computing and Storage 

Operating System

64-bit Windows 10

Controller Data Storage

27.3 TB

Expandable RAID Storage

Add an additional 32.7 TB of storage with Incustore

Controller Memory

64 GB

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Discover which live-cell imaging and analysis instrument works best for your lab.

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SX5 Resources

Featured Resources


Live-Cell Analysis 6th Edition Handbook

Real-time live-cell analysis is redefining the possibilities and workflows of cell biology. See how.

Download eBook

Label-Free Quantification of Cell Growth Using AI and Advanced Analyti...

Explore how an integrated AI-driven approach provides accurate measurements of proliferation across a range of cell types

Watch the Webinar

Advances in Live-Cell Biological Image Analysis

Enable more objective and quantitative analysis, leading to deeper insights while supporting scientific research at scale.

Download eBook

Product Resources


PDF | 238.6 KB

Incucyte® — Reagents, Consumables and Software

PDF | 8.8 MB
Incucyte - Empower Live-Cell Analysis Inside Your Incubator

Live-Cell Analysis Systems Brochure

PDF | 10.1 MB

FAQ for Live-Cell Imaging and Analysis Instrument: Incucyte® SX5


Incucyte® SX5 - Leverage up to five different fluorescence channels, up to three at a time (Green/Orange/Near-IR) plus HD Phase imaging, for long term time-lapse experiments. There is also the option of separately purchasing a Green/Red Optical Module or the Incucyte® SX5 Metabolism Optical Module, for use with the Incucyte® ATP Assay.

All models of the Incucyte® provide multi-user support with unlimited, free user licenses. Additionally, multiple assays can be run and imaged in different channels in parallel. However, only the Incucyte® SX5 and S3 support independent vessel scheduling for different image acquisition frequencies.

The Incucyte® itself is not an incubator but sits within a standard tissue culture incubator so cells are maintained in a physiologically relevant environment. For the Incucyte® SX5, an incubator larger than 200L is required.

The Incucyte® SX5 is our only instrument that, in addition to our phase channel, offers up to three fluorescent channels at one time with our Green/Orange/Near-IR Optical Module. This is a user swappable module that can be interchanged with the Incucyte® Green/Red Optical Module or the Incucyte® SX5 Metabolism Optical Module, for use with the Incucyte® ATP Assays. The Incucyte® SX5 also has an automated turret for the 4X/10X/20X objectives, as well as supporting independent scheduling of plates.

What to Expect from our Virtual Demo  

Because we want to provide the best and most relevant support to your research, Sartorius experts are available to demonstrate the features and benefits of our live-cell imaging and analysis systems in a personalized manner. 

  • Initial consultation with your local support team to understand your interests
  • Virtual demo at no cost to you!
  • Total demo time would be 2-4 hours, depending on number of applications

See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data.

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