Ambr® 15 - Advanced Microbioreactor System
For Cell Line Selection and Early Process Development
Ambr® 15 Cell Culture is a high throughput, automated bioreactor system for 24 or 48 parallel cultivations at the 10 –15 mL microbioreactor scale. This platform enables selection of the best clones, media and feeds under small-scale bioreactor conditions, ensuring a seamless scale-up. Implemented in Cell Line Development (CLD) laboratories worldwide, Ambr® 15 Cell Culture makes experimentation more cost effective by saving on labor, facility space, capital, media and consumables.
Modernize CLD workflows
Leave shake flasks and shaking plates behind to increase cell productivity and ensure robust performance.
Maximize throughput
With up to 48 bioreactors running in parallel one operator can screen more clones and conduct multi-factor DOE studies.
Reduce experiment turnaround time
Single-use bioreactors save setup time, reducing staff costs and turnaround time.
Improve consistency through automation
Liquid handling automation reduces variation and risk of human error.
Simplify clone ranking
A one-year license for Ambr® Clone Selection software is included, simplifying ranking analysis.
Parallel Operation of up to 48 Microbioreactors
The Ambr® 15 Cell Culture system includes single-use vessels, an automated workstation and powerful software. Installed in a biosafety cabinet for aseptic operation, Ambr® 15 monitors and controls 24 or 48 microbioreactor cultures in parallel to provide a reliable microscale model for upstream processes. Implemented in CLD workflows of major pharma and biologics companies as well as research and academic institutes.
Ambr® 15 Cell Culture Generation 2
Ambr® 15 Generation 2 Design
With an improved design, this next generation system offers better performance, increased process flexibility and expanded capabilities to support a wide range of upstream applications.
Automated Workstation
Automation of Parallel Experiments Offers Unparalleled Control
Fully automated parallel processing, monitoring and control with:
- New features: improved liquid handling, new culture station design, flexible deck layout and rapid vessel drain
- Standard and cooled workstation configurations
- Individual set point, monitoring and closed-loop control of pH and DO
- Independent control of O2, CO2 and N2 for each microbioreactor
- User-defined temperature and stirring set points for each culture station
- Extended low speed stirring range down to 150 rpm
Culture Stations
Flexibilty Within and Across Culture Stations
The culture stations are located on the workstation deck: two culture stations in a 24-way system, four in a 48-way system – each station holds 12 microbioreactors.
Temperature and stirring speed are consistent within a single culture station but can vary between different culture stations.
pH, DO, gassing, liquids feeds and samples are controlled independently for each individual bioreactor within a culture station.
Microbioreactors
A Microscale Preview of Lab-Scale Potential
Ambr® 15 microbioreactor vessels mimic the characteristics of lab scale bioreactors to enable optimal cell growth, productivity and product quality.
- 10 – 15 mL working volumes
- Single-use pH and DO sensors
- Integrated pitched blade impeller
- Port for liquid additions and for sampling
- Vessels with sparge tube for gassing into the impeller mixing zone, or without for headspace gassing
- Vessels available with temperature compensation for lower temperature applications
Control Software
The Power to Plan and Execute Ambr® 15 Experiments
Ambr® 15 control software supports:
- Easy experiment construction
- Effective process monitoring and control
- Detailed recording and data analysis with audit trail
- New software steps for media mixing, passaging and rapid vessel drain operations
The Ambr® 15 software includes a license for MODDE® DOE to assist with experiment planning and execution.
Process parameters and other variables can be tagged in Ambr® 15 to facilitate DOE, enabling easy transfer and analysis of results in MODDE®.
Modes of Operation
Ambr® 15 Cell Culture Modalities
Ambr® 15 Cell Culture is a microscale bioreactor model that reduces bottlenecks and accelerates development across a range of upstream applications. With the ability to efficiently explore different modes of operation, it is easy to investigate how cell lines and clones behave under batch, fed-batch and perfusion-mimic conditions, while the capability of media exchanges supports development of processes for cell and gene-based therapies.
Clone Selection
Highly Productive Clones With Optimal Quality Starts With Selection
Assessing clone performance in small-scale bioreactors ensures results translate at scale. Ambr® 15 Cell Culture combined with Ambr® Clone Selection for clone ranking optimizes workflows to reduce cell line development timelines and increase productivity.
Media and Feed Optimization
Streamline and Simplify Media and Feed Evaluation
Optimization of media and feeds can improve cell growth and productivity. Ambr® 15 Cell Culture control software enables the creation of media blends in plates on the deck or directly in the microbioreactors. Automated liquid handling saves time and reduces risk of error.
Early Process Parameter Definition
High Throughput Experimentation Alleviates Major Development Bottlenecks
Protocols can rapidly be established for both stable and transient cell lines. Parallel operation allows testing of multiple factors to define process parameters early in development, while Design of Experiments (DOE) embedded in the Ambr® 15 software facilitates the multifactorial experimental design and analysis.
Perfusion Mimic
Screening Under Perfusion Mimic Conditions
Ambr® 15 Cell Culture’s Rapid Vessel Drain option removes supernatant from settled cultures directly from the microbioreactors quickly and efficiently. The Ambr® 15 software includes novel process steps, such as cell bleeding based on cell count data, to automate perfusion mimic protocols.
Applications
Developing novel, complex processes quickly is essential to manufacture products on time and to specifications. Ambr® 15 Cell Culture helps to establish protocols for stable and transient cell lines, select suitable clones, and optimize media and processes to facilitate scale up to larger bioreactors.
Analyzer Options
Ambr® Analysis Module
Integration of the Analysis module with Ambr® 15 for at-line pH measurements automates bioreactor sensor calibration and in-process pH checks, while the Analysis module with BioPAT® Spectro allows connection to selected 3rd party Raman Spectrometers for spectroscopic analysis of bioreactor samples.
Integrated Cell Counters
The Ambr® 15 Cell Culture system can connect directly to the Beckman Vi-CELL™ XR or Roche Cedex HiRes for cell count measurements, or to the Nova Biomedical BioProfile® FLEX2 for cell counts and metabolite analysis. Samples transfer through a sample line to the integrated analyzers, and measurement results transfer back to the Ambr® 15 software for monitoring and process control.
Data-Connected Analyzers
The Octet® Label-free Detection Systems and Waters™ BioAccord™ LC-MS can connect to Ambr® 15 to transfer bioreactor sample data to the analyzer and return results to the Ambr® 15 software. Samples are manually transported between the systems.
Details
An Interactive Overview of the Ambr® 15 Cell Culture Workstation
Click the signs to learn more about the features that make the Ambr® 15 Cell Culture the bioreactor system of choice for Cell Line Development laboratories worldwide. This technology enables the selection of the best clones, media and feeds under small-scale bioreactor conditions for a seamless scale-up, all while saving on labor, facility space, capital expenditures, media and consumables.
Product Demo
Ambr® 15 Cell Culture Workstation
Ambr® 15 Cell Culture Microbioreactor
Learn More About Ambr® 15 Cell Culture
Ambr® 15 Downloads
Explore brochures, posters and application notes on Ambr® 15 for a full understanding of how the lab-scale bioreactor can increase productivity while reducing timelines across a number of applications.
Highlight Assets
Related Assets
Application Notes
Application Note
Approaches to Establishing a Perfusion Mimic Process in Ambr® 15 Cell Culture Generation 2
PDF | 338.2 KB
Application Note
Scalable MSC Suspension-Based Process Adaptation and Optimization in Ambr® 15 Cell Culture Microbioreactors Using DOE
PDF | 3.0 MB
Application Note
Scalable Upstream PD for the Suspension-Based Production of LVs for CAR-T Cell Therapies with Multiparallel and Benchtop Bioreactor Systems and DOE Methodology
PDF | 1.4 MB
Application Note
Accelerating Process Optimization by Combining Ambr® 15 Cell Culture with Octet® Titer Measurements
PDF | 2.9 MB
Application Note
Direct LC-MS Characterization of GlycoformDistribution and Low Molecular Weight Impurities in mAb Process From Ambr® 15 Bioreactors
PDF | 757.8 KB
Posters
Poster
Investigating the Use of Automation and Integrated Analytics to Enhance Cell Line and Process Development Capabilities
PDF | 2.6 MB
Poster
Optimization of the HEK293 Suspension Cultivation With a DoE-Approach in the Ambr® 15 Bioreactor
PDF | 3.1 MB
Poster
Demonstrating Scalable T-Cell Expansion in Stirred-Tank Bioreactors Using Ambr® 15 Cell Culture
PDF | 1.8 MB
Poster
Approaches for Applying Perfusion Mimic in Ambr®15 for Increased Understanding of Intensified Processes at the Microscale
PDF | 471.5 KB
Poster
A Rapid, Low-risk Approach for Process Transfer of Biologics from Development to Manufacturing Scale
PDF | 1.4 MB
Poster
