Biolayer Interferometry for Label-Free Biomolecular Interaction Analysis

For over 20 years, we have dependably supported our customers by creating established fluidic-free Biolayer Interferometry (BLI) platforms, consumables, and software. These products are trusted by scientists worldwide to support their bioanalytical needs for protein interaction analysis spanning from drug discovery to antibody engineering to bioprocessing and quality control (QC).

Our dedication to quality is reflected in an install base of more than 3,500 customers and is well-documented in more than 7,000 customer publications.  Over the years, our top priority has been providing exceptional technical and scientific support, ensuring you can quickly and effectively utilize our products to achieve your experimental goals.

We continue to shape the future with Octet®, the only brand currently combining both industry-leading label-free technologies – Biolayer Interferometry (BLI) and Surface Plasmon Resonance (SPR) for real-time label-free measurements of binding kinetics and affinity or to determine the active concentration of an analyte.

BLI Solutions

Performance, flexibility and confidence for real-time label-free, high-quality binding kinetics and analyte quantitation.

Octet®️ BLI systems offer an advanced, fast, robust, and fluidics-free approach for protein-protein and protein-small molecule analysis. The Octet®️ BLI platform allows direct detection of specific proteins or drug molecules - even in complex mixtures and unpurified samples such as cell culture supernatants and lysates. 

Always opt for the genuine Octet® platform to empower your research by enabling faster time to market, quicker project completion and greater project capacity.

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Biolayer Interferometry (BLI) Instruments

The Octet® BLI is an easy-to use, real-time analytical tool that rapidly screens critical quality attributes.

2-Channel System: Octet® R2

Grows With Your Biomolecule Characterization Needs

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Octet R4 label free detection system

4-Channel System: Octet® R4

The Perfect Balance Between Cost and Throughput with Given Leeway

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Octet R8 label free detection system instrument

8-Channel System: Octet® R8

Unmatched Flexibility and Versatility in Protein Analysis

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Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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Octet RH16 ultra high throughput label free detection system

16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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Octet N1 label free detection system

Personal Assay Octet® N1 System

Affordably priced label-free analysis for individual researchers on any budget

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Explore BLI Consumables, Software and Support

Octet biosensors

Biosensors & Kits

Available in a wide range of surface chemistries for use in a diverse set of biomolecular applications.

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GxP Compliance

GxP Compliance Tools

A wide range of compliance tools for developing validated assay methods on the Octet® BLI platform.

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Octet® Systems Software

Facilitate data acquisition and data analysis in laboratories working under GMP, GLP and 21 CFR Part 11 regulations.

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Octet® System Service and Support

Receive outstanding assistance for both technical issues and assay-related enquiries.

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BLI Promotions

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Global - Get up to $190K (€160K) off when you bundle Octet® BLI and SPR systems

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20 Years of BLI Expertise

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Two Decades of Supporting Groundbreaking Scientific Research

Proven countless times – the genuine Octet® BLI is an industry-leading technology with more than 7000 peer reviewed publications covering a large variety of research areas and applications. The Octet® Publication Database provides an easy way to search scientific publications that reference and/or cite the use of our Octet® products for label-free biomolecular interaction analysis in real time. 

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Octet® Publication Database

Literature references covering a large variety of research areas and applications

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Break Free from Bargain Imitations — See What Genuine Octet® BLI Users Are Saying

Thousands of researchers worldwide use Octet® systems in diverse fields ranging from drug discovery to biopharmaceutical development and academic research to determine binding specificity, active analyte concentration, affinity, or binding kinetics in real-time.

Discover what users of genuine Octet® BLI have to say about this indispensable tool that has helped them advance their research and accelerate the development of new therapeutics and diagnostics.

Testimonials

Easy to use, speedy and robust.

Application Area: High-throughput antibody characterization and small molecule analysis

"The Octet® RH96 lets our team carry out quantitation at high-resolution that is comparable to an HPLC and rapid throughput. It allows shortening of our experimental timelines and less cost for cell line development projects. Our team uses it for label-free, real-time data generation and it's a facile instrument to use that provides superior quality data at a very cost-efficient price." 

- Sankalp Gupta, Sanofi

Really wonderful instrument definitely can't live without it!

Application Area: High throughput mAb characterization and small molecule analysis

"We use it for label-free, real-time data generation. The Octet® RH96 is a facile instrument to use and provides superior quality data at a very cost-efficient price."

– Kesh Prakash, Emergent BioSolutions

The ease of use, robustness, speed, and costs make this an exemplary instrument.

Application Area: HTS drug development screening tool

"The Octet® RH96 allows quantitation with high resolution comparable to an HPLC and rapid throughput. The platform allows shortening of our timelines and less cost for cell line development. The instrument is very robust, easy to use and is well supported by an experienced support staff of application scientists, software engineers, service engineers and sales. Integration of the instrument into an automation system was completely supported by the vendor."

– Glenn Yamasaki, Bayer Healthcare

Would buy that instrument again.

Application Area: IgG Quantitation in Cell Line Development

"Easy to use instrument [Octet® R8], good results. Easy to explain to new users. Great support."

– Julius Grzeschik, Ferring Pharmaceuticals

First choice for interaction studies.

Application Area: Drug discovery

"Octet® is very easy to use system for fast screening of protein - small molecule interaction studies. Easy to maintain and definitely value for the money because of time saved in measurements time."

– Juha Määttä, Tampere 

Great results, can’t live without this instrument!

Application Area: Drug discovery

"State of the art technology"

– Hailan Yao, Roskamp Institute

Octet® Performance: Sensitive, Clean, and Reproducible Data

Baseline Stability, Low Noise, and Low Baseline Drift are Key for High-Quality Data

The Octet® R Series offers an advanced, fluidics-free approach to bimolecular analysis, with a wide variety of off-the-shelf biosensors for rapid binding kinetics and quantitation analysis. These systems utilize Sartorius’ genuine label-free Biolayer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like crude cell culture supernatants and lysates.

The Octet® BLI systems monitor binding events in real time to calculate on rates (ka), off rates (kd) and affinity constants (KD). The superior sensitivity enables measurement of low molecular weight molecules down to 150 Da. The following example shows the kinetic interaction analysis of a small molecule.

Figure 1. The kinetic analysis of between a ligand, biotinylated-carbonic-anhydrase, and the small molecule analyte Furosemide (330.7 Da) with Super Streptavidin (SSA) Biosensors at 1000 rpm and 25°C on an Octet® R8 system. Furosemide working solutions were prepared in serial dilutions of 0.1, 0.3, 1.0. 3.0, 10.0 µM (n=4), respectively. Data was processed and curve fitted using a 1:1 binding model. 

High Resolution and Low Noise to Measure High Affinity Interactions 

Slow off-rate measurements show minimal changes in signals in the dissociation step requiring a combination of stable baselines and low baseline drifts for accurate measurement of the dissociation phase.

In addition, capturing measurable dissociation of the interaction may take hours of (> 2 hr data acquisition) specifically at the dissociation phase of higher affinity interactions that exhibit very slow off rates. Therefore, measuring longer dissociation times is also critical for measuring high-affinity interactions.

The Octet® R8 system simultaneously enables measurements of up to 8 samples in a single unattended run. Combined with a unique microplate cover that keeps samples intact by minimizing evaporation, it allows scientists to collect accurate measurements with evaporation control for up to 12 hours.

The measurement of one high-affinity interaction measured in triplicates in a long dissociation experiment on the Octet® R8 system is demonstrated below. Full kinetic characterization of an antibody-antigen interaction was run three times with a total run time of 11 hours.

Figures 2 and 3 provide two examples of long dissociation measurement over several hours to allow the detection of very slow off-rates.

Figure 2. A high-affinity interaction between an IgG and Fab measured in triplicates that required a long dissociation step time of more than three hours was measured using an Octet® R8 system. The total experiment run time was 11 hours for measurements made in triplicate.

Figure 3. Kinetics of high-affinity interaction between Human Epidermal Growth Factor (HER2) protein and anti-HER2 monoclonal antibody (Herceptin) analyzed in the Octet® R8 system demonstrating reliable high-resolution kinetic data.

Educational Resources

7000+ Octet publications New
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Octet® Publication Database

Literature references covering a large variety of research areas and applications

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Brochure

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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Case Study

Bispecific Antibodies: From Research to Clinical Application

Learn how Octet BLI transforms our approach to drug discovery and development

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Webinar

Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

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Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Octet® BLI Products are Confidently Adopted in Regulated Environments


Not Just GxP Compliance Tools — Rely on a Comprehensive Toolbox

From antibody quantitation, ligand binding characterization to potency assays, Sartorius offers a wide range of GxP compliance tools for developing validated assay methods on the genuine Octet® BLI platform.

The United States Pharmacopeia (USP) has recently released a new general chapter <1108> which for the first time cites the use of biolayer interferometry in key ligand binding applications that provide more precise kinetic data and that can measure a relatively wide range of binding affinities. Read More

Explore Octet® Maintenance and Validation Services

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Installation Qualification (IQ)

Verifies and documents all necessary components. Software required for operation are received and properly installed in accordance with Sartorius installation protocols.

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Operational Qualification (OQ)

Tests critical subsystems of the instrument including optical system, shaker, and temperature controller to verify operating functions are in accordance with the Sartorius product operational specifications.

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Performance Qualification (PQ)

Test performance of the system in the customer environment with pre-validated assays to verify instrument operating in accordance with a written and pre-approved specifications document by Sartorius.

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Preventative Maintenance (PM)

The system lamps are replaced, calibrated, inspected for any potential issues, and proactively addressed. A comprehensive multipoint inspection and testing ensures each system meets the Sartorius product operational specifications.

Sartorius Offers Octet® 21 CFR Part 11 Software and a Full Line of GxP Products and Services as Part of the Octet® GxP Package Including Our Biosensor Validation Support Services

This service enables the Octet® BLI users to sample multiple lots of a biosensor during assay qualification and validation and reserve a well-characterized lot for purchase. Hence, customers can order up to five different biosensor lots, up to 20 trays from each lot for evaluation, and reserve up to 40 additional trays for future purchase.

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Approved Drugs Utilizing Octet®️ BLI Data in Their Regulatory Approval Process

Octet®️ BLI assays can help identify potential safety concerns and improve the accuracy of drug evaluations. Here are several examples of companies, including Pfizer, AstraZeneca, or Roche, who have utilized such assays to generate data submitted as part of the supporting information for the drug’s approval with regulatory bodies such as the FDA or the EMA.

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World-Class Octet® Customer Support

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We Prioritize Providing Exceptional Customer Support to Ensure a Seamless Experience for Our Valued Customers

Our commitment to this is demonstrated through our:

  • Live Global Technical Support — Live support is available via phone and email, ensures prompt responses within 24-48 hours.
  • Knowledge Base — We understand the importance of troubleshooting and have developed the Octet® Knowledge Base, a comprehensive resource available anytime to assist users in resolving assay-related challenges. 
  • Software Access — Our customers can conveniently obtain the software they need, enhancing their experience with our products.
  • Safety Datasheets & Certificates — Recognizing the significance of timely information, we have centralized Octet® safety datasheets and certificates of analysis, making them readily available online for immediate download.

Experience the Octet® Difference

Meet Some of Our Technical Support Team

Yixin Lin Ph.D, Manager, Global Technical Support

Yixin Lin Ph.D, Manager, Global Technical Support

Helping Octet® BLI and SPR users for the past 13 years to navigate biosensor assay development, technical support, and troubleshooting and more than 20 years of experience in customer-facing environments. Yixin joined the Octet® team in 2010 as a Field Applications Scientist and is an expert in label-free biosensor assays, kinetics, immunoassays, biomarker proteomics, and statistics. Yixin leads a team of highly specialized scientists that provide world-class support to Octet® customers. Yixin enjoys taking walks, appreciating nature and city streets in her spare time.

Terence Hui, M.Sc. Manager, Field Applications Scientist

Terence Hui, M.Sc. Manager, Field Applications Scientist 

Terence is a dedicated hands-on protein biochemist and leads a highly skilled team of applications scientists in the Americas. He has been with the Octet® family for over 12 years and has been committed to advancing technical innovation, bringing extensive well-rounded experience in biological science, protein characterization, biosensor assay development, and people management. Outside of work Terence likes to explore new food, listens to music, and hiking.  

Hendrik Wuensche, PhD, Technical Support Scientist

Hendrik Wuensche, PhD, Technical Support Scientist

Hendrik has been supporting and guiding Octet® users in Europe to build SPR and BLI assays for more than 11 years. He is an expert in label-free biosensor assays, kinetics, quantitation, epitope binning, and drug discovery screening. Hendrik joined the Octet® team in 2012 as an Applications Scientist and has recently joined the technical support team supporting Octet® users globally. Hendrik likes mountain biking and caring for his bonsai trees in his spare time.

Tim Heiseler, Ph.D, Senior Application Scientist

Providing scientific customer support in the field of label-free interaction analysis for over 10 years across various European regions, Tim joined the Octet® team in 2013 as a Field Application Scientist. He has expertise in label-free interaction analysis, including kinetics and quantitation, as well as immunoassays, high-throughput screening, protein engineering and design. Tim is an integral member of the experienced BioA FAS EMEA team at Sartorius BioA, offering localized scientific support to their customers.

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Frequently Asked Questions on Biolayer Interferometry (BLI)

Biolayer Interferometry (BLI) is a label-free technology for measuring biomolecular interactions. It is an optical analytical technique that has gained popularity in the fields of biochemistry, biology, and medical research due to its real-time, high-throughput analysis capabilities.

Biolayer Interferometry (BLI) is a label-free analytical technique commonly used to study biomolecule interactions. It is based on the principles of optical interferometry, where changes in the thickness of a biological layer constructed on a biosensor tip cause shifts in the interference pattern of white light reflected from the biolayer. This shift in the interference pattern correlates to real-time layer thickness changes, which allows for monitoring molecular binding and dissociation.

BLI is especially useful in providing quantitative data on the analyte concentration, binding kinetics, binding affinity, and specificity of biomolecular interactions without requiring fluorescent or radioactive labeling. This makes it a popular choice in various fields, such as drug development, antibody testing, and protein analysis.

In BLI, a biosensor tip is dipped into a solution containing biomolecules. These molecules bind to the biosensor, forming a thin biological layer. The interference pattern changes as more molecules bind to the biosensor, causing a shift in the wavelength of the reflected light. By monitoring this shift, scientists can track the binding process in real-time. This allows them to measure the rate of association (ka) and dissociation (kd), as well as the binding affinity (KD) of the interaction, providing valuable insights into the biomolecular interaction of a wide variety of molecules.

Biolayer Interferometry (BLI) offers several advantages over other biomolecular binding analysis methods such as Surface Plasmon Resonance (SPR) and Enzyme-Linked Immunosorbent Assay (ELISA). Here are some of the key benefits:

  1. BLI is a label-free technique that eliminates potential interference from labeling, allowing for the analysis of unmodified biomolecules.
  2. BLI provides real-time analysis of biomolecular interactions, including binding kinetics, affinity, and concentration.
  3. Compatibility with Diverse Sample Types: BLI can be used with a range of sample types, including crude samples or samples in complex matrices, without significant interference
  4. Requires minimal or no sample preparation, which is beneficial for high-throughput screening.
  5. Wide Range of Applications: BLI can be used to study various interactions, including protein-protein, protein-DNA/RNA, protein-small molecule, and even cell-based interactions. This versatility makes it applicable in many research and development contexts.
  6. Low Sample Consumption: BLI typically requires a small sample and can be reused, which is advantageous when working with precious or limited materials.
  7. Ease of Use and High Throughput: BLI instruments are generally user-friendly and capable of handling multiple samples simultaneously, making them suitable for high-throughput screening applications.
  8. Cost-Effectiveness: Compared to some other techniques, BLI can be more cost-effective, especially in terms of reagent use and maintenance of the equipment.

Biolayer Interferometry (BLI) has great potential in various fields, including biomedical research, drug discovery, and diagnostic development. It is a highly effective tool for antibody characterization and for exploring interactions between proteins and small molecules, which can significantly aid in therapeutic development.

BLI can be useful in biosimilar production, assessing vaccine efficacy, and quality control for protein therapeutics. It is also beneficial in studying cellular signaling and gene regulation through interactions between ligands and receptors, and nucleic acids and proteins. In the biopharmaceutical industry, BLI can play a critical role in ensuring quality control and can help in the development of diagnostic tools for detecting disease biomarkers, highlighting its wide-ranging applications in life sciences.

BLI biosensors operate on the principle of interferometry employing a white light source, which emits light across a broad range of wavelengths. The interference between the light reflected from the top of the biological surface on the sensor tip and the light reflected from an internal reference layer creates an interference pattern. This pattern is captured by a detector, typically a spectrometer or photodetector, and is used to measure changes in the interference signal. These changes in the interference pattern are directly related to the biomolecular interactions occurring on the sensor surface.

Biolayer Interferometry is a powerful and versatile technology that provides a valuable tool for studying biomolecular interactions. Its ability to provide real-time, label-free analysis makes it an invaluable tool in advancing the understanding in biochemistry, biology, and medical research, contributing significantly to the development of new drugs and therapies.

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