Octet^® BLI Resource Library

Harmonizing Regulatory Guidelines for Assay Validation

Current regulatory guidelines for assay validation were harmonized for kinetic-based assay to derive a common set of parameters.

Label-free to Decipher CRISPR/Cas Mechanisms

Octet^® BLI to measure and characterize CRISPR/Cas mediated binding and Gene activation

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

Small-Molecule Interaction Discovery and Characterization

With Octet^® BLI Workflows

Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet^® Platform

Determining Nanoparticle Vaccine Antigenic Efficiency

Nanoparticle (NP) vaccine and antigen delivery platforms have emerged as a promising approach due to their ability to interact with immune components

Stability, Formulations and Aggregation Studies

Expanding Octet^® Applications in Downstream Biologics Characterization

Accurate Measurements on the Octet^® Platform

Without Added Sample Purification Steps

Advancing Development of Coronavirus Vaccine and Therapeutics

BLI Technology Plays a Vital Role in the Race for a SARS-CoV-2 Treatment

Recent Insights into Covid-19 Binding Epitopes

Researchers are using the Octet^® system to understand the virus's binding mechanisms as a first step in the development of a vaccine

Modernize Biopharmaceutical Quality Control Testing

Quality control of biological products to support clinical trials and post market assay activities require the evaluation of multiple critical product...

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

Ligand Binding Assays That Meet Compliance with Octet^® BLI Systems

Octet^® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...

Characterizing Membrane Protein Interactions

By Octet^® Bio-Layer Interferometry (BLI) Platforms

A Simple Method to Determine Relative Potency (EC₅₀)

Using Octet^® BLI Technology

Simplifying Biotherapeutic Manufacturing and Quality Control

With Label-Free Biosensor Technology

Advances in Immunogenicity Evaluation - Paving the Way for Safer Biolo...

Anti-drug antibodies (ADA) can modify the drug’s pharmacodynamics (PD) and/or pharmacokinetics (PK), making it crucial to detect them through an immun...

Optimizing Kinetics Assays to Avoid Avidity Effects

A fundamental understanding of the differences between affinity and avidity is essential when designing and developing assays

Rapid, Automated, At-Line Adenovirus Quantitation Using Octet^® BL...

Learn how we report the development of a rapid, high-throughput capsid assay for AV quantification on the Octet^® BLI platform that demonstrates excell...

Octet^® R4 Bio-Layer Interferometry Platform SARS-CoV-2

Biophysical Characterization of the Binding of SARS-CoV-2 to the ACE2 Protein Using the Octet^® R4 Bio-Layer Interferometry Platform

Quantitation of Fc-Fusion Protein in Cell Culture Supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein in crude cell culture supernatants.

Octet^® AAVX Biosensors

For Rapid and Direct Quantitation of AAV Capsids

Measure Pharmacokinetics of Monoclonal Antibodies

Scientists from Elanco^® use Octet^® BLI to rapidly design and establish a preclinical PK method for analyzing canine antibodies

Customized Quantitation of Recombinant Therapeutic Proteins

Using Octet^® SAX Biosensors

Analysis of FcRn-Antibody Interactions on the Octet® Platform

Assay design recommendations and best practices for producing the highest quality of kinetic data.

Cross Competition or Epitope Binning Assays on the Octet® RH96 System

Epitope binning through cross competition assays is a powerful tool in evaluating functional diversity in antibody clones.

Analysis of Fc Gamma Receptor IgG Interactions on Octet^® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

Second Generation Anti-Human IgG Fc Capture Biosensors

For Affinity Characterization of Human IgG-Antigen Interactions and Quantitation of Human IgGs or Human Fc Region Containing Proteins 

Mannose Glycans Content Screening for Human-mAb Samples

A novel approach for relative mannose content screening and ranking of human monoclonal antibody (mAb) samples using the Octet^® GlyM Kit

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

Validated Quantitation and Activity Assay of FaB

For Process Development and Quality Control

MAb Quantitation Protein A HPLC vs Protein A Bio-Layer Interferometry

Rapid, accurate and cost-effective quantitation of monoclonal antibodies (MAbs) is essential for bioprocessing.

Potency Assay Development Qualification and Validation Strategies

A method for evaluating the binding of an Fc gamma receptor III (FcγR) molecule to the widely characterized NIST mAb.

Screen Bispecific Antibody Using Protein A and His1K Biosensors

Rapid quantitative screening of bispecific antibodies using Protein A and HIS1K biosensors

Accelerating Process Optimization in Cell Line Development

This application note describes the power of using the Octet^® platform along with Ambr^® 15 Cell Culture to identify optimum yield conditions.

A Fast and High Precision Influenza Vaccine Potency Assay

An important understanding of vaccine development process performance, and for correctly scaling each process step.

Octet^® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

Rapid and Efficient Measurement of Critical Quality Attributes and Par...

How Are Label-Free Technologies Used in Early Drug Discovery?

Modern analytical techniques designed for real-time analysis and high-throughput capabilities significantly reduce the time to the discovery

Label-Free Detection

Technologies, Key Considerations, and Applications

Accelerating the Development and Manufacture of Therapeutics

Establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032 requirements

Overcoming the Challenges of Infectious Diseases

Explore the latest methodologies and strategies in the development of future vaccines fighting infectious diseases with label-free approaches

Accelerating Vaccines and Virus Research

Live Cell Analysis, Advanced Flow Cytometry and Label-Free Binding Analysis

Addressing the Challenges of QC Labs in Vaccine Manufacturing

In cell culture-based vaccine manufacturing processes, microbial contamination control and impurity testing are crucial elements of quality control.

Advancing the Development of COVID-19 Neutralizing Antibodies

Learn more about how virus-neutralizing antibodies have proven to be a key tool in the fight against SARS-CoV-2.

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

AAV Capsid Titer Data 80% Faster Compared to ELISA

Bioprocess Five Additional Batches of AAV Samples Annually

Understanding Covid-19 Vaccine and Therapeutic DevelopmenT

Learn more about how Octet^® systems are used to advance Coronavirus research and vaccine development

Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly sca...

Octet^® Bio-Layer Interferometry (BLI) Technology

Label-free alternative for characterizing protein-protein and drug-protein interactions in standard microtiter plate format

High Productivity and Process Economy in GxP Applications

Quantitation assays must show precision, accuracy, linearity, and reproducibility

Octet^® BLI Systems

Unmatched Versatility for Discovery, Development and Quality Control

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

Octet^® AHC2 Biosensors

For Quantitation and Kinetic Characterization of Human Fc-Region Containing Proteins

High Precision Streptavidin 2.0 (SAX2) Biosensors

Streptavidin is often deployed as the molecule of choice for covalent linking to solid surfaces for ligand binding assays applications.

Octet® BLI Quantitation Assays: Method Development Guideline

Design and develop biological assays that conform to USP <1032> for accuracy, precision, linearity and limit of quantitation (LOQ) to be establi...

CHO Host Cell Protein Detection

Designed for a rapid high-throughput quantitation of residual CHO Host Cell Proteins (HCPs) in bioprocessing samples during production and development...

Octet^® ProL Biosensors

For Quantitation of Whole-Molecule Antibody and Antibody Fragments

A New Wave Biosensor Approach for AAV Process Development

REGENXBIO Inc. shares their experience in finding a high-throughput technique for AAV capsid concentration quantification.

Affinity versus Avidity Webinar

The multivalent nature of antibodies can lead to avidity effects that skew binding affinity data. Hear from a protein analytics expert about avoiding...

Antibody-Drug Conjugates (ADCs) Webinar

Simplifying the Characterization of the drug trastuzumab to Kadcyla^®️ and Enhertu^®️

Reduce Cycle Times for AAV Quantification

See the Octet^® AAVX Biosensor in comparison to an in-house assay on the Octet BLI platform.

CHO for Accelerated Antibody Development

Explore applications of the CHO antibody simultaneous display and secretion technology

Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Host Immunity

Cell surface Nucleocapsid (N) protein may play an important role in host adaptive immunity to SARS-CoV-2

Rapid Characterization and Selection of SARS-CoV-2 Vaccine Candidates

Using Bio-Layer Interferometry Technology, we developed a set of assays to identify antibodies with SARS-CoV-2 reactivity

Bio-Layer Interferometry as a Strategic Platform

To validate covalent proximity inducing small molecules with synthetic tumor immunotherapeutic applications

Novel High-Throughput Glycoanalytics for Biopharmaceuticals

Improve the overall drug discovery process to increase the success rate of preclinical candidates.

Integrating Octet^® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

Characterizing Critical Reagents in Support of Drug Development

BLI technology for affinity characterization antibodies can benefit critical reagent workflows

Fragment Based Drug Discovery of Co-Factor Inhibitors for p97

Virtual screening of molecular databases and biochemical/biophysical techniques

Optimers™️ - Future Ready Antibody Mimetics

Discover the new versatile Octet^®️ R series and the use of Optimer™️ binders to improve affinity purification of novel biologics

Drug Discovery with High-throughput Biomolecular Interaction Analysis

Learn how Octet^® BLI works in therapeutic discovery and development, including common challenges and solutions

Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

Automating Octet® Assays for Ligand Screening

Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of high-performanc...

The End of End-Point Assays

Faster time-to results allows assessment of accurate and precise data earlier in the workflow

Investigating Synthetic Immune Recruitment

by Proximity Inducing Molecules

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

Enhanced Productivity and Labor Efficiency

In Lot Release and In-Process Testing of Biologics in GxP Laboratories