Customized Quantitation of Recombinant Therapeutic Proteins Using High Precision Octet® Streptavidin Biosensors (SAX)
The accurate determination of recombinant protein titer is critical to the selection of high-producing clones during cell line development and in optimization of bioreactor conditions during production of therapeutic proteins.
Concentration measurements for these therapeutic proteins are often done using enzyme-linked immunosorbent assays (ELISAs), RP-HPLC or coomassie-stained SDS-PAGE gels.
Although these techniques are prevalent, they are encumbered by drawbacks that include long assay times, extensive hands on and in some cases, low throughput. In addition, techniques such as ELISA tend to exhibit high variability, resulting in lower accuracy.