The Use of High-Throughput Flow Cytometry and Bio-Layer Interferometry in Virus Research

Join us for an engaging virtual seminar consisting of two presentations from Public Health England (PHE) and The Pirbright Institute. Learn about the role of high-throughput flow cytometry and bio-layer interferometry in virus research. Topic titles and abstracts include:

Measuring Antibody-Dependent Complement Deposition to Assess Immunity to SARS-CoV-2
(i) As the world reeled from the impact of the COVID-19 pandemic, professionals began crafting laboratory assays on the subject of natural immunity and vaccine design and testing. While the presence of neutralisation antibodies has been the presumed correlate of protection from infection, other antibody functions have been linked to protection from severe disease. We will discuss the challenges of developing and running a magnetic bead-based antibody-dependent complement deposition assay on the iQue® platform and show its utility in measuring functional immunity in convalescent and vaccine samples.

Investigating Avidity of Antibodies Against the Capsid of Foot-and-Mouth Disease Virus Using Bio-Layer Interferometry
(ii) Foot-and-mouth disease (FMD) is a high-consequence disease of cloven-hoofed livestock caused by the FMD virus (FMDV), with vaccine-afforded protection largely correlating with antibody titre. However, this relationship is not absolute and there is an increasing appreciation of the role played by antibody avidity. Because of this, characterizing antibody avidity has important consequences in regards to vaccine development. Here, we used bio-layer interferometry to characterize the avidity of monoclonal and polyclonal antibodies to the surface of FMDV particles. A peptide mimicking a receptor-binding loop was used, along with recombinantly expressed virus-like particles (VLPs),to display the entire viral epitope landscape and reflect the complete repertoire of antibody virus binding.


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