Best Practices for Pipetting 3D Cell Culture Hydrogels

Application Note

Three-dimensional (3D) cell cultures are of increasing interest in research areas such as stem-cell based therapies and drug discovery. However, preparing 3D cell cultures can be difficult, and poor pipetting technique may lead to high variation in 3D cell culture experiments.

Pipetting and mixing viscous hydrogels and sensitive cells are especially challenging. Picus®️ Nxt pipettes are ideal for the preparation of hydrogels as they enable standardization of the sample preparation workflow, erasing the potential negative impact of personal pipetting practices. For 3D cell cultures such as spheroid applications, GrowDex®️ is an excellent choice of hydrogel: it is easy-to-use, animal-free, and has no batchto-batch variation. In contrast to many other hydrogels, GrowDex®️ does not require polymerization, thus allowing it to be handled at room temperature—making the product easy to work with and promoting reproducible results.

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