Vivaspin 20 VS2001
Ultrafiltration unit with a MWCO of 10,000 Dalton and a unique twin membrane (polyethersulfone), suitable for starting volumes up to 20 ml for sample concentration with high process flexibility: centrifugation or centrifugation of pressurised units, as well as pressure filtration in stationary benchtop mode.- High flux PES membrane
- High retentate recovery > 90 %
- Vertical twin membrane for ultra fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Volume graduation for easy reading/monitoring
- 100-fold concentration with twin vertical membranes
- Additional option for pressurized operation
Description
Vivaspin 20 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. "Pressure-fugation" is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.
Rotor Compatibility
Please note: Vivaspin 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes
Performance Characteristics
| Time to concentrate up to 30x [min.] at 20°C and solute recovery % | ||||
|---|---|---|---|---|
| Mode | Centrifuge | Centrifuge | Bench top | Press-fuge |
| Rotor | Swing bucket | 25° Fixed angle | Pressure | Swing bucket |
| Sart volume | 20 ml | 14 ml | 10 ml | 10 ml |
| Min./Rec. | Min./Rec. | Min./Rec. | Min./Rec. | |
| Cytochrome c 0.25 mg/ml (12,400 MW) | ||||
| 3,000 MWCO PES | 110 / 97 % | 180 / 96 % | 60 / 96 % | - / - |
| BSA 1.0 mg/ml (66,000 MW) | ||||
| 5,000 MWCO PES | 23 / 99 % | 29 / 99 % | 50 / 98 % | 14 / 98 % |
| 10,000 MWCO PES | 16 / 98 % | 17 / 98 % | 32 / 97 % | 8 / 97 % |
| 30,000 MWCO PES | 13 / 98 % | 15 / 98 % | 32 / 97 % | 8 / 97 % |
| IgG 0.25 mg/ml (160,000 MW) | ||||
| 30,000 MWCO PES | 27 / 97 % | 20 / 95 % | 46 / 94 % | 13 / 97 % |
| 50,000 MWCO PES | 27 / 96 % | 22 / 95 % | 46 / 93 % | 13 / 96 % |
| 100,000 MWCO PES | 25 / 91 % | 20 / 90 % | 42 / 88 % | 12 / 94 % |
| Latex beads 0.004% in DMEM +10% FCS (0.055 μm) | ||||
| 300,000 MWCO PES | 20 / 99 % | 35 / 99 % | 10 / 99 % | - / - |
| Latex beads 0.004% in DMEM +10% FCS (0.24 μm) | ||||
| 1,000,000 MWCO PES | 4 / 99 % | 12 / 99 % | 4 / 99 % | - / - |
| Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
| 0.2 μm PES | 15 / 95 % | 5 / 95 % | 20 / 95 % | 2 / 95 % |
Product properties
| Pack size |
12
|
| MWCO |
10.000 Dalton
|
| Membrane |
Polyethersulfone (PES)
|
| Type |
Centrifugal unit / pressure driven unit
|
Technical attributes
| Swing Bucket Rotor |
20 ml
|
| Fixed Angle Rotor |
14 ml
|
| With Pressure Head |
15 ml
|
| Width |
30 mm
|
| Active Membrane Area |
6.0 cm²
|
| Filtrate vessel |
Polycarbonate (PC)
|
| Body |
Polycarbonate
|
| Concentrator Cap |
Polypropylene (PP)
|
| Pressure Head / Pressure-fuge Head |
Acetal / Aluminium
|
| Hold-up volume |
<20 µl
|
| Overall length |
*With pressure head
|
| Membrane material |
Polyethersulfone (PES)
|
| Dead stop volume |
50 µl
|
Application Note
Brochure
-
Broch_Ultrafiltration_Family_SLU1508-e.pdf (1.647 KB)
Manual
-
Manual_Vivaspin6+20_SLU6092-e.pdf (477 KB)
Catalogue
-
Cata_Integrated_Lab-Equipment_S--0300-e.pdf (9.537 KB)
-
Cata_Lab-Filtration_SLU0006-e.pdf (3.713 KB)
Equipment
Vivacell 250 Accessories
| VCA012 | 4 mm o.d. pressure tubing (3 m) | on request | |
| VCA011 | Male coupling | on request | |
| VCA010 | Female coupling | on request | |
| VCA002 | Air Pressure Controller (APC) | on request |
Accessories for Vivaspin
| VSA005 | Vivaspin 20 Diafiltration Cups, 12/Pack | on request | |
| VCA200 | Vivaspin 20 Pressure Head | on request |
Vivacell 70 Accessories
| VCA005 | Charge valve for Pressure-fuge head | on request |


