Vivaspin 20

Vivaspin 20 VS2001

Ultrafiltration unit with a MWCO of 10,000 Dalton and a unique twin membrane (polyethersulfone), suitable for starting volumes up to 20 ml for sample concentration with high process flexibility: centrifugation or centrifugation of pressurised units, as well as pressure filtration in stationary benchtop mode.
  • High flux PES membrane
  • High retentate recovery > 90 %
  • Vertical twin membrane for ultra fast concentration
  • Universal rotor compatibility
  • Safe sample concentration due to dead-stop volume
  • Convenient concentrate recovery by pipetting
  • Volume graduation for easy reading/monitoring
  • 100-fold concentration with twin vertical membranes
  • Additional option for pressurized operation

Description

Vivaspin 20 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. "Pressure-fugation" is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.

Rotor Compatibility

Please note: Vivaspin 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes

Performance Characteristics

  Time to concentrate up to 30x [min.] at 20°C
and solute recovery %
     
Mode Centrifuge Centrifuge Bench top Press-fuge
Rotor Swing bucket 25° Fixed angle Pressure Swing bucket
Sart volume 20 ml 14 ml 10 ml 10 ml
  Min./Rec. Min./Rec. Min./Rec. Min./Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)        
3,000 MWCO PES 110 / 97 % 180 / 96 % 60 / 96 % - / -
BSA 1.0 mg/ml (66,000 MW)        
5,000 MWCO PES 23 / 99 % 29 / 99 % 50 / 98 % 14 / 98 %
10,000 MWCO PES 16 / 98 % 17 / 98 % 32 / 97 % 8 / 97 %
30,000 MWCO PES 13 / 98 % 15 / 98 % 32 / 97 % 8 / 97 %
IgG 0.25 mg/ml (160,000 MW)        
30,000 MWCO PES 27 / 97 % 20 / 95 % 46 / 94 % 13 / 97 %
50,000 MWCO PES 27 / 96 % 22 / 95 % 46 / 93 % 13 / 96 %
100,000 MWCO PES 25 / 91 % 20 / 90 % 42 / 88 % 12 / 94 %
Latex beads 0.004% in DMEM +10% FCS (0.055 μm)        
300,000 MWCO PES 20 / 99 % 35 / 99 % 10 / 99 % - / -
Latex beads 0.004% in DMEM +10% FCS (0.24 μm)        
1,000,000 MWCO PES 4 / 99 % 12 / 99 % 4 / 99 % - / -
Yeast 1.0 mg/ml (S. Cerevisiae)        
0.2 μm PES 15 / 95 % 5 / 95 % 20 / 95 % 2 / 95 %

Product properties

Pack size
12
MWCO
10.000 Dalton
Membrane
Polyethersulfone (PES)
Type
Centrifugal unit / pressure driven unit

Technical attributes

Swing Bucket Rotor
20 ml
Fixed Angle Rotor
14 ml
With Pressure Head
15 ml
Width
30 mm
Active Membrane Area
6.0 cm²
Filtrate vessel
Polycarbonate (PC)
Body
Polycarbonate
Concentrator Cap
Polypropylene (PP)
Pressure Head / Pressure-fuge Head
Acetal / Aluminium
Hold-up volume
<20 µl
Overall length
*With pressure head
Membrane material
Polyethersulfone (PES)
Dead stop volume
50 µl

Equipment

Vivacell 250 Accessories

VCA012 4 mm o.d. pressure tubing (3 m) on request
VCA011 Male coupling on request
VCA010 Female coupling on request
VCA002 Air Pressure Controller (APC) on request

Accessories for Vivaspin

VSA005 Vivaspin 20 Diafiltration Cups, 12/Pack on request
VCA200 Vivaspin 20 Pressure Head on request

Vivacell 70 Accessories

VCA005 Charge valve for Pressure-fuge head on request
Order number:

VS2001

Amount: more less
Price: on request
Send request

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