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Sartorius AG
Weender Landstr. 94-108
37075 Goettingen, GermanyPhone: +49.551.308.0
Fax: +49.551.308.3289
Sartobind® Phenyl Jumbo 96HICP42E3ESS
- The first Membrane Adsorbers for HIC
- Simple handling
- Fast operation
- For capturing and polishing
- Less buffer consumption
Description
Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. On average 50% of a protein or peptide surface is accessible for hydrophobic interaction. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.
Product properties
| Bed volume |
5.000 mL
|
| Membrane area |
18,2 m²
|
| Pack size |
1
|
| Application |
Capturing and polishing
|
| Membrane |
Hydrophobic interaction chromatography membrane (HIC)
|
| Bed height |
8 mm
|
Technical attributes
| Reusable |
Yes
|
| Pore size |
3 - 5 µm
|
| Bed height |
8 mm
|
| Maximum Venting Pressure |
0.5 bar, 0.05 MPa, 7 psi
|
| Material |
Polypropylene, polyester
|
| Housing material |
Polypropylene (PP)
|
| Chemical compatibility |
Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
|
| Thermal stability |
For Sartobind phenyl devices, pretreatment with sodium hydroxide is recommended instead of autoclaving.
|
| Biosafety |
All materials of this product meet the requirements of the current United States Pharmacopoeia (USP) Biological Reactivity tests for Plastic Class VI (Systemic Injection, Intracutaneous and Implantation tests).
|
| Membrane material |
Stabilised reinforced cellulose
|
| What you receive |
1 capsule, 1 operating instruction, 1 certificate
|
| Ligand |
Phenyl
|
| Ligand density |
3 µeq/cm²
|
| Dimensions |
30,2 x 85 cm
|
| Typical dynamic binding capacity 10% per unit |
72,8 g
|
| Recommended flow rate |
25 l/min
|
| Void volume |
7 l
|
| Maximum Pressure |
0,3 MPa
|
| Connectors |
Sanitary 1 - 1 1/2"
|
| pH stability |
2 - 14 (short term), 3 - 13 (long term)
|
| Reference protein |
Gamma globulin from bovine blood
|
| Membrane type |
Hydrophobic interaction chromatography membrane (HIC)
|