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Vivaflow 50


  • Plug and play laboratory cross flow cassette
  • Modular - scalable to 6 units
  • Disposable
  • Volumes from 0.1 l -3 l
  • Optimal for aqueous sample concentration e.g. proteins and nanoparticles
  • High flow rate and recovery


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Description

Vivaflow 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm². One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm² module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow 50 package comes with two cassettes, tubing, flow restrictor, one series interconnector and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running more than two Vivaflow 50 devices in parallel and series

For speeding up the concentration, as many as six Vivaflow 50 cassettes can easily be connected using 2 T-connectors for connecting 2 Vivaflow cassettes at the inlet and at the outlet and series interconnectors for connecting the devices inbetween. A pressure indicator needs to be built in the inlet for optimal control.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of multiple cassettes
  • Economical and disposable product

Unique in its performance

A single 50cm² module will typically reduce 500 ml to less than 15 ml in under 50 minutes. Less than 10 ml minimum system recirculation for highest concentrations. Less than 1.5 ml non recoverable hold up volume. Near total recoveries achievable with a single 10 ml rinse.

Performance Characteristics

  Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
     
  Single device Three devices Solute recovery %  
  250 ml start vol. min. 1 L start vol. min. Direct 10 ml rinse
BSA 1.0 mg/ml (66,000 MW)        
5,000 MWCO PES 34 49 96 % > 99 %
10,000 MWCO PES 22 32 94 % > 99 %
30,000 MWCO PES 22 32 92 % 99 %
50,000 MWCO PES 20 29 92 % 98 %
γ Globulins 1.0 mg/ml        
100,000 MWCO PES 43 62 92 % 98 %
100,000 MWCO RC 40 58 92 % 98 %
Yeast 1.0 mg/ml (S.Cerevisiae)        
0.2 μm PES 33 47 92 % 98 %

Overall length107 mm
Overall height84 mm
Overall width25 mm
Channel width15 mm
Channel height0,3 mm
Active Membrane Area50 cm²
Hold-up volume1,5 ml
Minimum Recirculation Volume< 10 ml
Non-recoverable Hold-up< 0,5 ml
Pump Flow200 - 400 ml/min
Operating pressure max.3 bar
Max. temperature60 °C
Main HousingPolycarbonate (PC)
Flow ChannelTPX (PMP)
Membrane supportTPX (PMP)
Seals and O-ringsSilicone
Pressure indicatorPolypropylene, SS spring
Flow RestrictorPolypropylene (PP)
FittingsNylon (NY)
TubingPVC (medical grade)
S_Export_UFYes

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Vivaflow 50

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  • Plug and play laboratory cross flow cassette
  • Modular - scalable to 6 units
  • Disposable
  • Volumes from 0.1 l -3 l
  • Optimal for aqueous sample concentration e.g. proteins and nanoparticles
  • High flow rate and recovery

Manuals

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