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Vivaflow 200


  • Plug and play laboratory cross flow cassette
  • Re-usable
  • Volumes from 0.5 l -5 l
  • Optimal for cell culture supernatant and virus concentration
  • High flow rate and recovery


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Description

Vivaflow 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single rinse. Vivaflow 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Equipment required

The standard Vivaflow 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running 2 Vivaflow 200 cassettes in parallel

For speeding up the concentration, two Vivaflow 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows in parallel.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of 2 cassettes
  • Re-usable product

Performance Characteristics

  Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
     
  Start volume Average flux Recovery %  
  1 litre ml/min Direct 25 ml rinse
Lysozyme 0.25 mg/ml (14,000 MW)        
2,000 MWCO Hydrosart 160 6 97 % > 99 %
3,000 MWCO PES 180 5 97 % > 99 %
BSA 1.0 mg/ml (66,000 MW)        
5,000 MWCO PES 29 33 98 % > 99 %
5,000 MWCO Hydrosart 70 14 98 % > 99 %
10,000 MWCO PES 23 41 96 % > 99 %
10,000 MWCO Hydrosart 35 27 98 % > 99 %
30,000 MWCO PES 25 38 96 % 99 %
30,000 MWCO Hydrosart 20 48 96 % > 99 %
50,000 MWCO PES 22 43 96 % 98 %
γ Globulins 1.0 mg/ml (average 160,000 MW)        
100,000 MWCO PES 54 18 96 % 99 %
100,000 MWCO RC 45 21 96 % 99 %
Yeast 1.0 mg/ml (S. Cerevisiae)        
0.2 μm PES 11 86 92 % 98 %
Dilute solute concentration, start volume
1 litre at 3 bar, 10,000 MWCO PES
       
BSA 0.001 mg/ml 18 52 90 % 98 %
BSA 0.01 mg/ml 20 47 92 % 98 %
BSA 0.1 mg/ml 21 45 94 % 99 %
Start volume 5 litres (two VF200 in
parallel at 3 bar) 10,000 MWCO PES
       
BSA 1.0 mg/ml (66,000 MW) 67 70 97 % > 99 %

Overall length126 mm
Overall height138 mm
Overall width38 mm
Channel width10 mm
Channel height0,4 mm
Active Membrane Area200 cm²
Hold-up volume5,3 ml
Minimum Recirculation Volume< 20 ml
Max. temperature60 °C
Non-recoverable Hold-up< 2 ml
Pump Flow200 - 400 ml/min
Operating pressure max.4 bar
Main HousingAcrylic
Flow ChannelAcrylic
Membrane supportPolypropylene (PP)
Seals and O-ringsSilicone
Pressure indicatorPolypropylene, SS spring
Flow RestrictorPolypropylene (PP)
FittingsNylon (NY)
TubingPVC (medical grade)
S_Export_UFYes

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Vivaflow 200

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  • Plug and play laboratory cross flow cassette
  • Re-usable
  • Volumes from 0.5 l -5 l
  • Optimal for cell culture supernatant and virus concentration
  • High flow rate and recovery

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