Tangential | Crossflow Concentrators

Disposable and reusable tangential | crossflow cassettes for concentration, diafiltration and buffer exchange of biological samples and nanoparticles from 0.1 – 5 l with MWCOs from 3 kDa to 1,000 kDa. Available with PES and Hydrosart® ultrafiltration membranes, Vivaflow 50, Vivaflow 50R, and Vivaflow 200 are the only dedicated laboratory crossflow cassettes in the market. Our tangential | crossflow concentrators are simple to set up and can be operated with any laboratory pump with flow rates of 200 - 400 ml/min.

Vivaflow 50

Vivaflow 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm². One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm² module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Vivaflow 200

Vivaflow 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single rinse. Vivaflow 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).